This product is sufficient for ≤10 assays. When reconstituted with 10 ml deionized water, each vial will yield a solution with a pH of approximately 7.3, containing the following components: 0.3 mM 4-Hydroxyazobenzene-2-carboxylic acid (HABA), 0.45 mg/ml avidin, 0.3 M sodium chloride (NaCl), 0.01 M N-[2-Hydroxyethyl]piperazine-NN-[2-ethanesulfonic acid] (HEPES), a buffer with a pKa of 7.5, 0.01 M magnesium chloride (MgCl2), and 0.02% sodium azide as a preservative.
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表单
lyophilized powder
质量水平
用途
sufficient for ≤10 assays
颜色
orange
适用性
suitable for spectrophotometric determination of biotin and biotin conjugates in a wide variety of samples
储存温度
2-8°C
一般描述
4-羟基偶氮苯-2-羧酸 (HABA) 是一种抗生物素蛋白结合染料，可通过生物素进行化学计量置换。A500 处的后续吸收更改为分光光度法测定生物样品中的生物素水平提供了基础。
HABA/抗生物素蛋白试剂对依赖于生物素定量的显色应用具有特异性。
应用
HABA/亲和素试剂适用于分光光度法测定多种样品中的生物素和生物素结合物。
试剂能够检测 μ 处的生物素摩尔浓度（即 1 nmol/ml）。通常用于定量生物素化化合物中的生物素。
重悬
在 pH 约为 7.3 的缓冲盐水中制备即用型 HABA/抗生物素蛋白溶液，复溶时加入防腐剂 10ml 去离子水。
免责声明
除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、 体外 诊断用途、 离体 或 体内 治疗用途或任何类型的消费或人类或动物应用。
相关产品
产品编号
说明
价格
危险声明
预防措施声明
危险分类
Aquatic Chronic 3
储存分类代码
13 - Non Combustible Solids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
A Einspanier et al.
Biology of reproduction, 61(2), 512-520 (1999-07-20)
Relaxin is a peptide hormone with a broad range of biological activities, related not only to parturition and lactation but possibly also to decidualization, implantation, and early pregnancy. The present study was designed to investigate the secretion pattern of relaxin
R S Niedbala et al.
Journal of biochemical and biophysical methods, 13(4-5), 205-210 (1986-11-01)
Parameters and conditions of an enzyme based assay for biotin and avidin are presented. Biotinylated glucose-6-phosphate dehydrogenase when complexed with avidin becomes inactivated. Thus it was possible to construct a competitive assay system for biotin. The assay is sensitive between
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I would like to know how many assays can be done with a VIAL KIT and how much material it contains,
1 answer-
Helpful?
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how much HABA is in the vial
1 answer-
When reconstituted to 10mL, the vial will contain 0.3 mM HABA. Based on the molecular weight of HABA, 242.23 gmol, the calculated mass per vial is 726.69 ug. Please see the link below to review additional informaition available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/378/451/h2153dat.pdfHelpful?
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