推荐产品
ligand
matrix surface
特點
autoclavable 20 min at 121°C
包裝
pack of 10 mL
製造商/商標名
Cytiva 17-0727-20
基質
polystyrene/divinylbenzene
樣品平均尺寸
15 μm (monosized)
cleaning in place
1-14
工作範圍
1-12
容量
~10 mg binding capacity
適合性
suitable for bioprocess medium
一般說明
應用
High quality assurance standards in combination with the patented production method, particle structure is consistent both bead-to-bead and batch-to-batch.
A controlled pore size distribution and large specific surface area offer excellent capacity for a wide range of molecules, from small peptides and oligonucleotides up to large Proteins. With full pH range stability, SOURCE™ 15RPC is a strong alternative to silica-based RPC matrices.
SOURCE™ 15RPC is available in a range of different bulk pack sizes for easy scale-up and process development. As member of the BioProcess media range, SOURCE™ 15RPC meets industrial demands with security of supply and comprehensive technical and regulatory support.
特點和優勢
- Mono-sized 15 μm rigid beads gives Low back pressures and high flow rates allowing for high productivity.
- Designed for high resolution reversed phase chromatography of Proteins, peptides and oligonucleotides.
- High chemical stability allowing for wide range of working conditions with good resistance to cleaning conditions at high pH
- BioProcess medium supported for industrial applications.
儲存和穩定性
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
商品
“Ghosting” in chromatograms due to poor eluent quality; trace organic impurities concentrate, causing unknown peaks.
The different stages of working with SOURCE™ (Cytiva) for high-resolution separation are discussed, from purification to polishing and scaling up
This page discusses column packing and preparation techniques for reverse phase chromatography.
This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.
实验方案
In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.
相关内容
Poor-quality eluent components can cause a phenomenon referred to as “ghosting”. Trace levels of organic impurities bind to the medium, concentrating during equilibration and sample application. When elution begins, these contaminants appear in the chromatogram as unknown, or “ghost” peaks.
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