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主要文件

GE17-0507-01

Sigma-Aldrich

Mono S® HR 16/10

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About This Item

分類程式碼代碼:
41115700
NACRES:
SB.52

ligand

-OCH2CH(OH)CH2OCH2CH(OH)CH2SO3-

材料

PEEK column

描述

Ion Exchanger Type (Methyl sulfonate strong cation)

包裝

pkg of 1 ea

參數

435 psi

床尺寸

16 mm × 100 mm

床體積

20 mL

柱內徑

16 mm

尺寸

基質

polymeric

基質活性組

methyl sulfonate phase

樣品平均尺寸

10 μm

cleaning

1-14

工作範圍

2-12

分離技術

cation exchange

一般說明

Mono S® HR 16/10 is a strong cation exchanger prepacked with MonoBeads. The column is an excellent choice for the polishing step in purification of proteins, peptides, and other biomolecules when high purity is required. The 20 mL bed volume of the column offer high capacity.

應用

  • Monodisperse 10 μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
  • Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q ion exchangers.
  • Compatible with ÄKTA® design, FPLC™ System, and other high-performance LC systems.
  • MonoBeads are also available as Mono P® for chromatofocusing

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

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法律資訊

FPLC is a trademark of Cytiva
Mono P is a registered trademark of Cytiva
Mono S is a registered trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

实验方案

MonoBeads: Purification of Milligram Quantities with Highest Resolution

This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

相关内容

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

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