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Merck

GE17-0300-10

Ficoll® PM 400

Cytiva 17-0300-10, pack of 100 g

别名:

Density gradient medium

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About This Item

分類程式碼代碼:
12352201
NACRES:
NA.25

形狀

solid
spray-dried

包裝

pack of 100 g

製造商/商標名

Cytiva 17-0300-10

顏色

White

溶解度

soluble

一般說明

High molecular weight sucrose-polymers formed by copolymerization of sucrose with epichlorohydrin. The molecules are highly branched and the high content of hydroxyl groups leads to very good solubility in aqueous media.
It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be attained

特點和優勢

  • For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.
  • Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution.
  • Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/mL can be achieved.
  • Better osmotic properties than sucrose.
  • Preserves functional and morphological integrity.
  • Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size (under conditions of sedimentation at unit gravity).
  • Does not penetrate biological membranes.
  • Serves as the raw material for preparation of Ficoll-Paque gradients.
  • Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Ficoll is a registered trademark of Cytiva

儲存類別代碼

13 - Non Combustible Solids


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商品

The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.

This page shows methods to remove Percoll from a sample after centrifugation.

Density Marker Beads facilitate Percoll gradient monitoring in biological separations.

The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.

实验方案

以下操作步骤已接受Ficoll-Paque PLUS评估,推荐用于分离正常血样以尽可能提高可重现性。

The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.

相关内容

The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.

This page shows methods to remove Percoll from a sample after centrifugation.

Data was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll for a particular cell or tissue type.

Density Marker Beads are dyed derivatives of Sephadex™. There are ten color-coded bead types, each with a specific density. They have been specifically formulated for use in Percoll gradients and will not work with other media. Using Density Marker Beads as an external marker facilitates monitoring of the gradient shape and range.

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