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F2878

Sigma-Aldrich

Ficoll® PM 70

Ficoll® PM 70

Type 70

别名:

聚（蔗糖-共-表氯醇）

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50 G
$434.00
250 G
$1,740.00

$434.00

国内现货，预计发货时间2025年4月10日详情

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50 G
$434.00
250 G
$1,740.00

About This Item

CAS号:
72146-89-5
MDL编号:
MFCD00131071
UNSPSC代码:
12352201
NACRES:
NA.25

$434.00

国内现货，预计发货时间2025年4月10日详情

获取大包装报价

类型

Type 70

质量水平

200

表单

powder

分子量

~70,000

颜色

white

溶解性

H2O: soluble 100 mg/mL, clear to slightly hazy, colorless to faintly yellow

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相关类别

一般描述

Ficoll® PM70是一种高分子量支化多糖聚合物，公认不含任何离子化基团。这种蔗糖聚合物由蔗糖与环氧氯丙烷共聚形成。

应用

Ficoll® PM 70已用于：
  • 用作比较研究中的大分子，评估其在玻璃化溶液中的效果
  • 用作卵母细胞玻璃化过程的玻璃化溶液补充剂
  • 用作人肺成纤维细胞培养的低血清培养基成分，促进形成拥挤环境

生化/生理作用

Ficoll 常用作玻璃化溶液的大分子成分。由于其密集的分支，Ficolls也可用作理想的近球形分子拥挤剂。
在细胞体积信号转导和蛋白质重折叠研究中，FicollTM PM 70用作大分子拥挤剂。[1]这一物质可用于组织工程和大分子构象研究，开发、评估和使用大分子拥挤 (MMC) 系统和配置。 Ficoll PM-70还可用于玻璃化研究。

其他说明

蔗糖的非离子合成聚合物。
为了全面了解我们针对客户研究提供的各种多糖产品，建议您访问我们的碳水化合物分类页面。

法律信息

Ficoll is a registered trademark of Cytiva

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000401688
0000356212
0000352976
0000334002
0000327569

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Y Georgalis et al.
Journal of colloid and interface science, 386(1), 141-147 (2012-08-23)
Ficoll PM70 is an important branched polysaccharide polymer with a variety of applications in biology and biophysics. The accepted picture for the aqueous solutions of this polymer is that of a polydisperse nearly spherical suspension with a molecular weight of
C M Checura et al.
Theriogenology, 67(5), 919-930 (2006-12-19)
This study was designed to evaluate vitrification procedures for in vitro matured bovine oocytes for efficient blastocyst production after warming, IVF and culture. A second goal was to replace serum as the macromolecular component of the vitrification solution, without compromising
M Al-Habori
The international journal of biochemistry & cell biology, 33(9), 844-864 (2001-07-20)
Macromolecular crowding has been proposed as a mechanism by means of which a cell can sense relatively small changes in volume or, more accurately, the concentration of intracellular solutes. According to the macromolecular theory, the kinetics and equilibria of enzymes
M B Burg
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 10(5-6), 251-256 (2000-12-23)
The non-ideal properties of solutions containing high concentrations of macromolecules can result in enormous increases in the activity of the individual macromolecules. There is considerable evidence that macromolecular crowding and confinement not only occur in cells, but that these are
Pascal Salvetti et al.
Theriogenology, 74(5), 847-855 (2010-06-24)
The cryopreservation of oocytes is valuable for the preservation of women's fertility and might also be an interesting tool to preserve animal genetic biodiversity but it is not often used because of the very poor fertility recovered after thawing, especially
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实验方案

用于梯度离心的Ficoll® 400

Ficoll 400®是由蔗糖和环氧氯丙烷共聚而成的一种高度分支化的聚合物。Ficoll 400®是完全非离子性的。由于其含有丰富的羟基，Ficoll 400®具有很强的亲水性和水溶性。

Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

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Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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