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Merck

G6142

Sigma-Aldrich

甘油激酶 来源于纤维单胞菌

lyophilized powder, 25-75 units/mg protein

别名:

ATP:甘油3-磷酸转移酶, 甘油激酶

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

lyophilized powder

比活性

25-75 units/mg protein

分子量

~128 kDa (by gel filtration)

成份

Protein, ≥60% biuret

儲存溫度

−20°C

一般說明

Research area: Cell Signaling

Glycerol kinase (GK) is part of the FGGY carbohydrate kinase family.

應用

Glycerokinase from Cellulomonas sp. has been used:
  • for determining the kinetic characteristics of human and trypanosomatid phosphofructokinases using an enzyme-linked kinetic assay.
  • to study the effect of sugar in fluorescence emission.
  • in 2-Arachidonoylglycerol-based fluorescence assay for DH-463, a fluorescent activity-based probe for monoacylglycerol lipase.

生化/生理作用

甘油激酶催化甘油的tge MgATP依赖性磷酸化以产生sn-甘油-3-磷酸,并且是甘油利用中的限速酶。 它也受到果糖-1,6-二磷酸的反馈调节。

物理性質

等电点:4.2
米氏常数:4.4 x 10-5M(甘油),4.3 x 10-4M(ATP)
抑制剂:对对氯汞苯甲酸,重金属离子(Pb++,Fe++,Hg++,Ag+
最佳pH:9.8(G-3-PDH系统),7.8(G-3-P氧化酶系统)最佳温度:500C
pH稳定性:pH 5.5 x 10.0 (25oC, 20hr)
热稳定性:低于40oC(pH 7.5,15min)
底物特异性:这种酶催化ATP的磷酰基部分末端的立体特异性转移
到甘油的一个伯羟基,
形成sn-甘油-3-P。酶具有最高的
甘油特异性,也能磷酸化二羟基丙酮
和甘油醛(表1,2)。Mg++ 基本上是反应必需的。
.

單位定義

一个单位为在pH 9.8和25℃下,在PK/LDH的偶联体系内每分钟将1.0 μmole甘油和ATP转化为L-α甘油磷酸盐和ADP。

外觀

冻干粉含磷酸盐缓冲盐和葡萄糖酸钠

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Peter M Fernandes et al.
The Biochemical journal, 476(2), 179-191 (2018-11-09)
Eukaryotic ATP-dependent phosphofructokinases (PFKs) are often considered unidirectional enzymes catalysing the transfer of a phospho moiety from ATP to fructose 6-phosphate to produce ADP and fructose 1,6-bisphosphate. The reverse reaction is not generally considered to occur under normal conditions and
Fei Ying et al.
Scientific reports, 14(1), 3922-3922 (2024-02-17)
The influence of lipid metabolism on tumorigenesis and progression has garnered significant attention. However, the role of Glycerol Kinase (GK), a key enzyme in glycerol metabolism, in Esophageal Carcinoma (ESCA) remains unclear. To further elucidate the relationship between GK and
N Zwaig et al.
Science (New York, N.Y.), 153(3737), 755-757 (1966-08-12)
Fructose-1 ,6-diphosphate is a feedback inhibitor of the catabolic enzyme, glycerol kinase, in Escherichia coli. A mutant was isolated which produced a desensitized enzyme. Glucose was no longer as effective in preventing the utilization of exogenous glycerol by cells which
Zhongya Qin et al.
Biomedical optics express, 9(7), 3373-3390 (2018-07-10)
The femtosecond laser ablation in biological tissue produces highly fluorescent compounds that are of great significance for intrinsically labelling ablated tissue in vivo and achieving imaging-guided laser microsurgery. In this study, we analyzed the molecular structures of femtosecond laser-ablated tissues
Purification and properties of glycerol kinase from Escherichia coli.
S I Hayashi et al.
The Journal of biological chemistry, 242(5), 1030-1035 (1967-03-10)

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