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G3293

Supelco

葡萄糖测定试剂

for use with enzymatic assay kits GAHK20, SCA20, FA20, SA20

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20 ML
$131.00
50 ML
$226.00

$131.00

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20 ML
$131.00
50 ML
$226.00

About This Item

UNSPSC代码:
12164500
NACRES:
NA.84

$131.00

请联系客服了解存货情况
获取大包装报价索取更多信息

分析物化学类别

sugars (glucose)

技术

photometry: suitable

杂质

≤0.005% Creatine phosphokinase, glutathione reductase, myokinase, NADH-oxidase,NADPH oxidase, phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase
<0.01% ATP-ase, myokinase

应用

food and beverages
general analytical

储存温度

2-8°C

相关类别

特异性

与以下底物发生交叉反应可导致活性 < 0.05%：果糖、葡聚糖、景天庚糖、阿拉伯糖、半乳糖、木糖、海藻糖、甘露糖、山梨醇、+/-岩藻糖或核糖。

应用

加入包装所示体积（20mL 或 50mL）的去离子水。
通过己糖激酶-葡萄糖-6-磷酸的酶法对食物和其他物质中的葡萄糖进行定量测定。

相关产品

产品编号
说明
价格

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000390450
0000390450
0000409790
0000409790
0000389931

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Anne Riemann et al.
PloS one, 6(7), e22445-e22445 (2011-08-06)
Tumor micromilieu often shows pronounced acidosis forcing cells to adapt their phenotype towards enhanced tumorigenesis induced by altered cellular signalling and transcriptional regulation. In the presents study mechanisms and potential consequences of the crosstalk between extra- and intracellular pH (pH(e)
Michele Bertazzini et al.
Frontiers in plant science, 7, 288-288 (2016-03-26)
Numerous angiosperms rely on pollinators to ensure efficient flower fertilization, offering a reward consisting of nourishing nectars produced by specialized floral cells, known as nectaries. Nectar components are believed to derive from phloem sap that is enzymatically processed and transformed
Daniel S Straus
Molecular cancer, 12, 78-78 (2013-07-20)
Inflammation is a well-known etiological factor for colorectal cancer, but mechanisms underlying the linkage between inflammation and cancer are incompletely understood. We hypothesized that two pro-inflammatory cytokines, TNFα and IL-17, might play a role in promoting colorectal carcinogenesis. Aerobic glycolysis
Mor Tsamir-Rimon et al.
The New phytologist, 229(3), 1398-1414 (2020-09-04)
Carbon reserve use is a major drought response in trees, enabling tree survival in conditions prohibiting photosynthesis. However, regulation of starch metabolism under drought at the whole-tree scale is still poorly understood. To this end, we combined measurements of nonstructural
Chao Zhang et al.
The Journal of animal ecology, 85(6), 1431-1441 (2016-10-26)
A key challenge for ecologists is to predict how single and joint effects of global warming and predation risk translate from the individual level up to ecosystem functions. Recently, stoichiometric theory linked these levels through changes in body stoichiometry, predicting
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实验方案

Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)

Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)

改良Boehenger法酶促检测α-葡萄糖苷酶(EC 3.2.1.20)

改良Boehenger法酶促检测α-葡萄糖苷酶(EC 3.2.1.20)

Enzymatic Assay of Cellulase

To standardize an enzymatic assay procedure of cellulase.

纤维素酶的酶学测定

该实验方案使用分光光度法测定纤维素酶活性。黑曲霉纤维素酶催化内-1,4-β-D-糖苷键的水解。

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Questions

1–8 of 8 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. For how many tests this reagent will be sufficient?

    1 answer

    1. This product is available in a 20 mL or 50 mL package size. The entire vial should be reconstituted in DI water as indicated by the package size. Each test requires 1 mL of this reagent. The 20 mL package size is sufficient to perform 20 test and the 50 mL pack size is sufficient to perform 50 tests.

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  4. How much of the initial must be reconstituted in 20 ml of DI water?

    1 answer

    1. The entire vial should be reconstituted to the volume of the package size. The G3293-20 mL with 20 mL of DI water and the G3293-50 mL with 50 mL of DI water.

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  5. Is it possible to adapt Product G3293, Glucose Assay Reagent, for use with a 96 well plate reader?

    1 answer

    1. In principle, it should be possible to adapt assay procedures using this reagent to 96-well plate readers. Volumes of reagent, water, standard, and unknown should be reduced to fit the capacity of the test wells, and kept in the same proportions as those used with a spectrophotometer. The volume reduction will likely be 1:4 or 1:5. With the shorter "pathlength" of a multiwell plate, the sensitivity of the assay would likely decrease.

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  6. What is the detection limit of Product G3293, Glucose Assay Reagent?

    1 answer

    1. This reagent should be able to detect glucose down to approximately 0.01 mg/mL using a spectrophotometer with a 1 cm path length.

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  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  8. Is magnesium required for Product G3293, Glucose Assay Reagent?

    1 answer

    1. Magnesium, an activator of hexokinase, is included in the assay reagent. There is no need to add a magnesium salt to the reagent before use.

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