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G2625

Sigma-Aldrich

明胶 来源于猪皮肤

gel strength ~175 g Bloom, Type A

别名:

Teleostean gelatine

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100 G
$119.00
500 G
$179.00
1 KG
$315.00

$119.00

国内现货，预计发货时间2025年4月08日详情

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100 G
$119.00
500 G
$179.00
1 KG
$315.00

About This Item

CAS号:
9000-70-8
EC 号:
232-554-6
MDL编号:
MFCD00081638
UNSPSC代码:
12352202
NACRES:
NA.61

$119.00

国内现货，预计发货时间2025年4月08日详情

获取大包装报价

生物来源

Porcine skin

质量水平

200

类型

Type A

表单

powder

浓度

65-85% (biuret)

技术

cell culture | mammalian: suitable

溶解性

H2O: soluble 50 mg/mL

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应用

建议将该产品以1-5 μg/cm2或0.5-50 μg/mL用作细胞培养的基质。 最佳的浓度的确取决于细胞类型以及应用和研究目标。

明胶已用于多种应用。 它可用于对细胞培养器皿进行包被以改善细胞的附着，加入至PCR以稳定Taq DNA，作为蛋白质印迹、ELISA和免疫化学中的封闭试剂，以及作为细菌学中用于物种分化培养基的组分。 作为一种生物相容性的聚合物，它已被作为递送载体用于活性生物分子的释放以及支架的生成用于组织工程应用。在制药工业中，明胶可用作悬浮和包封剂，以及其他应用。

组分

明胶存在于胶原蛋白中，是一种具有高平均分子量的水溶性蛋白质的异质性混合物。 通过在水中将相关的皮肤、肌腱、韧带、骨骼等进行煮沸来提取蛋白质。 A类明胶来自于酸固化组织。 B类明胶来自于石灰固化组织。

注意

干燥的明胶，当在室温下储存于密闭容器中时，将可保持多年不变。 当在空气存在时在100℃下加热时，它会溶胀变软，并分解成碳质物质并伴有吡啶碱和氨的释放。

制备说明

该产品源自于猪皮。明胶可溶于热水而非冷水。 它几乎不溶于大多数有机溶剂，如醇、氯仿、二硫化碳、四氯化碳、乙醚、苯、丙酮和油。 由Bloom凝胶计所测定的Bloom指数，可表示由已知浓度溶液而形成的凝胶的强度。 Bloom指数与平均分子量成正比。 猪皮明胶的Bloom指数变化于90至300 g之间。该产品具有175的凝胶强度。

储存分类代码

11 - Combustible Solids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000417220
0000417219
0000417220
0000417219
0000405513

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Questions

1–2 of 2 Questions  

  1. What is the molecular weight of this Gelatin?

    What is the molecular weight of this Gelatin?

    1 answer

    1. The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
      Bloom Number Average Molecular Mass (Da)
      50−125 (Low Bloom) 20,000−25,000
      175−225 (Medium Bloom) 40,000−50,000
      225−325 (High Bloom) 50,000−100,000

      Please see the link below to review the general datasheet for Gelatin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdf

      Helpful?

  2. What is the molecular weight of this Gelatin?

    1 answer

    1. The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
      Bloom Number Average Molecular Mass (Da)
      50−125 (Low Bloom) 20,000−25,000
      175−225 (Medium Bloom) 40,000−50,000
      225−325 (High Bloom) 50,000−100,000

      Please see the link below to review the general datasheet for Gelatin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdf

      Helpful?

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