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生物源
rabbit
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen ~80 kDa
物種活性
human
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
技術
western blot: 0.5-1 μg/mL using HEK-293T cells expressing human GRK2 or Jurkat cell lysate
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... ADRBK1(156)
一般說明
G protein-coupled receptor kinases (GRK2) belongs to the GRK family that includes seven known GRKs, termed as GRK1-7. It is also known as β-adrenergic receptor kinase-1 (β-ARK1) and ADRBK1. It regulates the activity of G protein-coupled receptors (GPCRs). GRKs facilitate the uncoupling of GPCRs from G proteins, and participate directly in receptor endocytosis and trafficking, and modulation of ERK/MAPK cascade by GPCRs. It phosphorylates the activated form of the β2-adrenergic receptor (β2-AR) and related GPCRs. The GRK2 activity is regulated by different mechanisms including phosphorylation by kinases such as PKC, Src, ERK1/2 and p38MAPK. Phosphorylated ERK inactivates GRK2 on Ser670 in a negative feedback mechanism.
免疫原
Synthetic peptide corresponding to amino acids 674-689 located at the C-terminus of human GRK2/βARK1, conjugated to KLH. The immunizing sequence is specific to GRK2 (not found in human GRK1, and GRK3-7). It is identical in dog GRK2 and highly conserved (single amino acid substitution) in rat, mouse, and bovine GRK2/βARK1.
應用
Anti-GRK2 antibody is suitable for western blots of cortical synaptoneurosomes to study the β-arrestin-1 and ERK protein expression. It is also suitable for western blot at a concentration of 0.5-1μg/mL using HEK-293T cells expressing human GRK2 or Jurkat cell lysate.
生化／生理作用
G protein-coupled receptor kinase 2 (GRK2) is involved in the pathogenesis of chronic heart failure.
外觀
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Ruxu Zhai et al.
International journal of molecular sciences, 23(5) (2022-03-11)
G-protein coupled receptor (GPCR) kinase 2 (GRK2) is upregulated in heart failure (HF) patients and mouse models of cardiac disease. GRK2 is a regulator of β-adrenergic receptors (βARs), a GPCR involved in ionotropic and chronotropic responses. We and others have
Petronila Penela et al.
Cellular signalling, 15(11), 973-981 (2003-09-23)
G protein-coupled receptor kinases (GRKs) are key modulators of G protein-coupled receptor signalling. Increasing evidence points to the occurrence of complex mechanisms able to modulate the subcellular localization, activity and expression levels of GRKs, revealing new functional interactions of these
Xiu-Rong Ren et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(5), 1448-1453 (2005-01-27)
Signaling through beta-arrestins is a recently appreciated mechanism used by seven-transmembrane receptors. Because G protein-coupled receptor kinase (GRK) phosphorylation of such receptors is generally a prerequisite for beta-arrestin binding, we studied the roles of different GRKs in promoting beta-arrestin-mediated extracellular
Adrenal GRK2 upregulation mediates sympathetic overdrive in heart failure
Lymperopoulos A, et al.
Nature Medicine, 13(3), 315-315 (2007)
J A Pitcher et al.
Annual review of biochemistry, 67, 653-692 (1998-10-06)
G protein-coupled receptor kinases (GRKs) constitute a family of six mammalian serine/threonine protein kinases that phosphorylate agonist-bound, or activated, G protein-coupled receptors (GPCRs) as their primary substrates. GRK-mediated receptor phosphorylation rapidly initiates profound impairment of receptor signaling, or desensitization. This
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