F6796

Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• 别名: Anti-FGFR-2
• MDL编号: MFCD02096345
• UNSPSC代码: 51111800
• NACRES: NA.41

属性

• 生物来源: rabbit
• 偶联物: unconjugated
• 抗体形式: affinity isolated antibody
• 抗体产品类型: primary antibodies
• 克隆: polyclonal
• 表单: buffered aqueous solution
• 分子量: antigen 115-125 kDa (doublet)
• 种属反应性: human
• 包装: antibody small pack of 25 μL
• 增强验证: recombinant expression; Learn more about Antibody Enhanced Validation
• 技术: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using trypsin-digested, human and animal tissue sections; western blot: 1:2,000 using extract of FGFR-2 transiently transfected cells
• UniProt登记号: P21802
• 运输: dry ice
• 储存温度: −20°C
• 靶向翻译后修饰: unmodified
• 基因信息: human ... FGFR2(2263)

说明

一般描述

Fibroblast growth factors (FGFs) belongs to the large tyrosine kinase family of growth factor receptors (17-38kDa). The FGF family consists of at least seventeen members designated FGF-1 through FGF-17 that bind to high affinity FGF receptors (FGFRs). Soluble, secreted or possibly cleaved forms of FGFR-1 and FGFR-2 have also been found in body fluids. They are glycosylated 110-150 kDa proteins that are constructed of an extracellular ligand binding region with either two, or typically three, immunoglobulin (Ig)-like domains and an eight amino acid "acidic box", a transmembrane region and a cytoplasmic split tyrosine kinase domain that is activated following ligand binding and receptor dimerization. The ligand binding site of all FGFRs is confined to the extracellular Ig-like domains 2 and 3.

特异性

The antibody detects human FGFR-2 (bek) in extracts of transfected cells.

The antibody specifically reacts with human FGFR-2; no reaction with FGFR-1 or FGFR-3 is detected.

免疫原

synthetic peptide corresponding to amino acids 362-374 of the extracellular region of human FGFR-2 (Bek).

应用

Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit has been used in immunoprecipitation and immunoblotting.

生化/生理作用

Fibroblast growth factors (FGFs) are potent physiological regulators normal development, wound healing and repair, angiogenesis, a variety of neurotrophic activities, in hematopoiesis as well as in tissue remodeling and maintenance. FGFRs exhibit overlapping recognition and redundant specificity. Signal transduction by FGFRs requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. Subsequent association with cytoplasmic signaling molecules leads to DNA synthesis or differentiation. The signaling and biological responses elicited by distinct FGFRs substantially differ. FGFR-2 is highly expressed in developing human tissues including the brain (preferentially in glial cells), choroid plexus, skin, lung, kidney and bone. It may be found in several anchorage-dependent cells, such as normal and malignant breast cancer cells.

外形

Solution in 0.01 M phosphate buffered saline,
pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

制备说明

Affinity isolated on a peptide-agarose column.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 10 - Combustible liquids
• WGK: WGK 2
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable
• 个人防护装备: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)