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Merck

D9163

Sigma-Aldrich

DL-二硫苏糖醇

suitable for electrophoresis, ≥99%

别名:

1,4-二巯基苏糖醇, DTT, 克莱兰氏试剂

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About This Item

线性分子式:
HSCH2CH(OH)CH(OH)CH2SH
CAS号:
分子量:
154.25
Beilstein:
1719757
EC號碼:
MDL號碼:
分類程式碼代碼:
12161504
PubChem物質ID:
NACRES:
NB.22

品質等級

化驗

≥99%

形狀

powder

反應適用性

reagent type: reductant

技術

electrophoresis: suitable

mp

40-44 °C
41-44 °C (lit.)

溶解度

H2O: 50 mg/mL, clear, colorless to very faintly yellow

正離子痕跡

heavy metals (as Pb): ≤10 ppm

吸收

≤0.04 at 280 at 0.02 M

儲存溫度

2-8°C

SMILES 字串

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI 密鑰

VHJLVAABSRFDPM-QWWZWVQMSA-N

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應用

用于将 −SH 基保持在还原态的绝佳试剂;定量还原二硫化物。在 SDS-PAGE 前,DTT 在样品缓冲液中可有效还原蛋白二硫键。DTT 还可用于还原交联剂 N,N′-双(丙烯酰)胱胺的二硫键桥以分解聚丙烯酰胺凝胶基质。DTT 的刺激性和毒性都低于 2-巯基乙醇。通常,使用 DTT (100mM) 所需的浓度是使用 2-巯基乙醇 (5% v/v, 700mM) 的浓度的七分之一。

分析報告

针对用于还原 SDS-PAGE 进行了测试

象形圖

CorrosionExclamation mark

訊號詞

Danger

危險聲明

危險分類

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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