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Merck
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D225

Sigma-Aldrich

4,5-二氨基荧光素二乙酸酯 溶液

5 mM in DMSO, ≥97% (HPLC)

别名:

DAF-2 DA

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About This Item

MDL號碼:
MFCD01866183
分類程式碼代碼:
41116100
NACRES:
NA.28

品質等級

100

化驗

≥97% (HPLC)

形狀

solution

存貨情形

not available in Japan

濃度

5 mM in DMSO

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

4,5-二氨基荧光素二乙酸酯(DAF-2 DA)溶液是可检测活细胞中一氧化氮(NO)的膜透过性荧光探针。它可用于研究牛主动脉内皮细胞(BAEC)中的NO合成。DAF-2 DA成像表明，纤毛上皮细胞比非上皮炎性细胞产生更多的NO。

應用

活细胞一氧化氮的细胞可透过性荧光检测剂。

法律資訊

DAF-2 由 Daiichi Pure Chemicals Co.,LTD. 生产。
美国专利号5,874,590

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

188.6 °F - closed cup

閃點（°C）

87 °C - closed cup

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Determinants of shear stress-stimulated endothelial nitric oxide production assessed in real-time by 4, 5-diaminofluorescein fluorescence
Qiu W, et al.
Biochemical and Biophysical Research Communications, 286(2), 328-335 (2001)
Increased nitric oxide production in nasal epithelial cells from allergic patients-RT-PCR analysis and direct imaging by a fluorescence indicator: DAF-2 DA
Takeno S, et al.
Clinical and Experimental Allergy, 31(6), 881-888 (2001)
Anne Marie W Bartosch et al.
Biophysical journal, 113(1), 101-108 (2017-07-13)
Nitric oxide (NO) is a regulatory molecule in the vascular system and its inhibition due to endothelial injury contributes to cardiovascular disease. The glycocalyx is a thin layer of glycolipids, glycoproteins, and proteoglycans on the surface of mammalian epithelial cells.
Rachel A Battaglia et al.
eLife, 8 (2019-11-05)
Alexander disease (AxD) is a fatal neurodegenerative disorder caused by mutations in glial fibrillary acidic protein (GFAP), which supports the structural integrity of astrocytes. Over 70 GFAP missense mutations cause AxD, but the mechanism linking different mutations to disease-relevant phenotypes
Zoltán Serfőző et al.
Cellular signalling, 30, 67-81 (2016-11-26)
Olfaction, a chemosensory modality, plays a pivotal role in the orientation and behavior of invertebrates. The central olfactory processing unit in terrestrial stylomatophoran snails is the procerebrum, which contains NO synthesizing interneurons, whose oscillatory currents are believed to be the

商品

感应染料

一氧化氮 (NO) 是神经元、内皮细胞和免疫系统中的信号转运分子。

Sensor Dyes

Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.

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