This kit provides sufficient reagents to perform 18 chloroplast isolation procedures, using 30 grams of starting plant tissue, as described in the protocol. Please see the link below to review the product datasheet:_x000D_
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/114/229/cpisopis-ms.pdf
$1,270.00
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一般描述
叶绿体分离试剂盒为从植物叶片中分离完整的叶绿体提供了有效的方法。该方法包括机械式细胞壁和细胞膜破坏、过滤法细胞碎片收集和叶片组织、离心法叶绿体分离、采用Percoll®分层液或梯度法法从破碎的叶绿体之中分离完整的叶绿体。
应用
在叶绿体细胞器研究中，叶绿体分离试剂盒可用于从植物叶片中分离叶绿体以便进行结构和功能研究。
特点和优势
- 专门配制的缓冲液 - 采用易于执行的简单、成熟实验方案，节省时间
- 可分离完整的叶绿体 - 适于研究碳同化、电子流和磷酸化、代谢转运或蛋白质靶向等过程
- 总细胞器蛋白质 - 适于进行功能性研究、代谢转运、蛋白质组谱分析和基因组应用
- 规格方便 - 节省时间，减少浪费
适用性
叶绿体分离试剂盒已采用菠菜、豌豆、生菜、卷心菜、莙达菜和烟草进行过测试。
储存分类代码
10 - Combustible liquids
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Juan Manuel Pérez-Ruiz et al.
The Plant cell, 18(9), 2356-2368 (2006-08-08)
One of the mechanisms plants have developed for chloroplast protection against oxidative damage involves a 2-Cys peroxiredoxin, which has been proposed to be reduced by ferredoxin and plastid thioredoxins, Trx x and CDSP32, the FTR/Trx pathway. We show that rice
Yayi Tu et al.
PloS one, 10(8), e0136210-e0136210 (2015-08-27)
Potato virus Y (PVY) is an important plant virus and causes great losses every year. Viral infection often leads to abnormal chloroplasts. The first step of chloroplast division is the formation of FtsZ ring (Z-ring), and the placement of Z-ring
Yuexia Wang et al.
BMC plant biology, 20(1), 96-96 (2020-03-07)
The perturbance of chloroplast proteins is a major cause of photosynthesis inhibition under drought stress. The exogenous application of 5-aminolevulinic acid (ALA) mitigates the damage caused by drought stress, protecting plant growth and development, but the regulatory mechanism behind this
Martha Sainz et al.
Physiologia plantarum, 140(1), 46-56 (2010-05-22)
Drought and heat stress have been studied extensively in plants, but most reports involve analysis of response to only one of these stresses. Studies in which both stresses were studied in combination have less commonly been reported. We report the
Jimena Cascales et al.
Life (Basel, Switzerland), 7(4) (2017-11-23)
The use of molecular markers with inadequate variation levels has resulted in poorly resolved phylogenetic relationships within Ilex. Focusing on southern South American and Asian species, we aimed at contributing informative plastid markers. Also, we intended to gain insights into
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