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产品名称
LB205, 98062329
生物来源
human blood
生长模式
Suspension
核型
Not specified
形态学
Lymphoblastoid
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
细胞系来源
Human lymphocytes, Xeroderma pigmentosum variant, EBV-transformed
细胞系描述
Derived from mononuclear cells from the peripheral blood of a 62 year old male. The patient was diagnosed with Xeroderma pigmentosum variant, illustrating that the disease is heterogeneous. T cells from the original sample have been used for UV irradiation studies and hprt mutant frequency. The cell line has been established by transformation with EBV. Fibroblasts from the same individual are designated XP11 BR.
应用
DNA repair deficiency studies, hprt mutation analysis
培养基
RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).
传代培养常规
Maintain cultures between 3-9 x 100,000 cells/ml dilute every 1-2 days to 3 x 100,000 cells/ml 5%
其他说明
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