SSE-5

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC代码: 41106514

属性

• 生物来源: fish embryo
• 包装: tube of 5 μg 95122021-DNA-5UG; pkg of vial of cells 95122021-1VL
• 生长模式: Adherent
• 核型: Modal no. 55
• 形态学: Epithelial-like
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Fish Sockeye Salmon embryo

细胞系描述

SSE-5 was initiated in 1964 from pooled embryos of Sockeye Salmon (Oncorynchus nerka). The cell line that is also known as Sockeye Salmon embryo-5, is susceptible to infectious pancreatic necrosis virus (IPNV), viral hemorrhagic septicaemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV). For replication of salmonid viruses SSE-5 cells should be grown at 18°C.

应用

Isolation of viruses from clinical samples, virus propagation

培养基

MEM (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 15-21°C; split every 10-14 days. Fish cell lines detach easily during transit if the culture is very young. After resuscitation split cells 1:3 and culture for at leas

其他说明

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.