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生物来源
Rat x Rat Hybridoma
包装
tube of 5 μg 89111412-DNA-5UG
pkg of vial of cells 89111412-1VL
ImmunoDon
Osborne-Mendel rat spleen
骨髓瘤
Y3Ag1.2.3
反应性
Anti-p21 Protein, Harvey Murine Sarcoma Virus
技术
cell culture | mammalian: suitable
同位素/亚型
IgG1
运输
dry ice
储存温度
−196°C
细胞系来源
Rat Y3Ag1.2.3 x Rat Osborne-Mendel spleen
细胞系描述
The antibody reacts to p21 encoded by Ha-MuSV v-ras gene. Cross-reacts to p21 encoded by Kirsten MuSV v-ras gene, p21 encoded by BALB-SV and 29kDa gag-ras fusion protein encoded by Ra-SV. Recognises p21 species in some uninfected rodent, carnivore and primate lines.
免疫原
Ha-MuSV transformed syngeneic NRK cells
培养基
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
传代培养常规
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells will semi-attach. Shake cells off or use trypsin to remove cells from flask
其他说明
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