SCC-4

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89062002, human tongue, squamous cell carcinoma. Epithelial morphology.

• UNSPSC代码: 41106514

属性

• 生物来源: human mouth
• 包装: tube of 5 μg 89062002-DNA-5UG; pkg of vial of cells 89062002-1VL
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Epithelial
• 产品: Epidermal keratins; 40 kD Keratin
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human tongue squamous carcinoma

细胞系描述

Derived from a human squamous cell carcinoma (SCC) of the tongue from a 55-year-old male. SCC-4 cells have been reported to form colonies in semi-solid medium, and are not induced to differentiate by anchorage deprivation. Growth is enhanced by use of a feeder layer of 3T3 swiss cells.

培养基

DMEM:HAMS F12 (1:1) + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + 0.4 μg/ml hydrocortisone

传代培养常规

Split confluent cultures 1:3 to 1:4, i.e., seeding at 3-5 x 10,000 cells/cm² using trypsin/EDTA; 5% CO₂; 37°C

其他说明

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