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Merck

H0396

Sigma-Aldrich

氢化可的松-水溶性

Hydrocortisone-Water Soluble
1 of 2 reviewers received a sample product or took part in a promotion

BioReagent, suitable for cell culture

别名:

环糊精包被的氢化可的松

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100 MG
$287.00

$287.00


预计发货时间2025年1月31日详情


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100 MG
$287.00

About This Item

MDL號碼:
分類程式碼代碼:
12352209
PubChem物質ID:
NACRES:
NA.77
生物源:
plant (wild yam)
形狀:
crystalline

$287.00


预计发货时间2025年1月31日详情


获取大包装报价

生物源

plant (wild yam)

品質等級

產品線

BioReagent

形狀

crystalline

技術

cell culture | mammalian: suitable

溶解度

H2O: 400 mg/mL

運輸包裝

ambient

儲存溫度

room temp

SMILES 字串

C[C@]12CCC(=O)C=C1CC[C@H]3[C@@H]4CC[C@](O)(C(=O)CO)[C@@]4(C)C[C@H](O)[C@H]23

InChI

1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1

InChI 密鑰

JYGXADMDTFJGBT-VWUMJDOOSA-N

一般說明

氢化可的松有可能在无血清培养基中诱导血脑屏障特性的形成。建议用于上皮和内皮粘附细胞培养应用。

應用

水溶性氢化可的松已被用作杜氏改良Eagle培养基(DMEM)/高糖培养基中的补充剂,以培养乳腺肺泡(MAC-T)细胞。这一物质还用于研究其对半规管上皮通过上皮钠通道吸收阳离子的影响。

包裝

包装尺寸取决于 氢化可的松

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Repr. 1A

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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P H Wirtz et al.
Heart (British Cardiac Society), 90(8), 853-858 (2004-07-16)
To investigate whether stimulated monocyte cytokine release and its inhibition by glucocorticoids differs between men and women. In vitro monocyte interleukin 6 (IL-6) and tumour necrosis factor alpha (TNFalpha) release after lipopolysaccharide stimulation were assessed with and without co-incubation with
Alexander Kanitz et al.
PloS one, 7(11), e49568-e49568 (2012-11-21)
Vascular endothelial growth factor A (VEGFA) plays a key role in the angiogenesis of human skin. Elevated levels of VEGFA are associated with several pathological conditions, including chronic inflammatory skin diseases and several types of skin cancer. In particular, squamous
Felipe Perozzo Daltoe et al.
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The aim of our study was to isolate populations of keratinocyte stem cells based on the expression of cell surface markers and to investigate whether the culture could affect their phenotype. keratinocytes from human oral mucosa were sorted based on
Yohan Choi et al.
Asian-Australasian journal of animal sciences, 25(1), 44-51 (2012-01-01)
Calcium ions play an important role in the establishment and maintenance of pregnancy, but molecular and cellular regulatory mechanisms of calcium ion action in the uterine endometrium are not fully understood in pigs. Previously, we have shown that calcium regulatory
Yohan Choi et al.
Biology of reproduction, 81(6), 1122-1130 (2009-07-31)
Calcium ions have been implicated in the establishment and maintenance of pregnancy, but the regulatory mechanisms of calcium ions in the uterine endometrium and conceptus are not well understood in pigs. Recently, we showed that TRPV6, a calcium ion channel

Questions

1–4 of 4 Questions  
  1. Dopo avre sciolto in acqua Hydrocortisone (H0396) a che temperatura deve essere conservato?

    1 answer
    1. Stock solutions may be refrigerated for up to 2 weeks. For long term storage, the stock solution may be stored at -20°C in aliquots for 6 - 12 months. Avoid repeated freeze/thaw cycles. This product is not sterile. Stock solutions should be filter sterilized using a 0.22 micron filter.

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  2. Which temperature would you suggest to store "400 mg/mL stock solution"? and for how long? Do you suggest to prepare H0396 in a sterile environment and further filter working solution using the 0.22um filter before cell culture use?

    1 answer
    1. Stock solutions may be refrigerated for up to 2 weeks. For long term storage we recommend -20 deg C in aliquots for 6 - 12 months. Avoid repleated freeze/thaw cycles. This product is not sterile. Stock solutions should be filter sterilized using a 0.22 micron filter.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. How do I calculate the molarity of product H0396, Hydrocortisone-Water Soluble?

    1 answer
    1. Product H0396 (Hydrocortisone-Water Soluble) is a complex of Hydrocortisone and cyclodextrin. Cyclodextrin is used to aid in the solubility of Hydrocortisone into water. In order to prepare a molar solution of Hydrocortisone you will have to base it on the Hydrocortisone content in the solution and not the entire complex.  Product H0396 is formulated to contain approximately 85 mg Hydrocortisone per gram of powder.  Based on this average, if you are preparing a 400 mg/ml stock solution, there will be approximately 34 mg of Hydrocortisone in 1 ml.  Below is the calculated molarity of Hydrocortisone when the complex is dissolved at 400 mg/ml in water. molecular weight X unknown molarity X volume = complex weight X complex ratio362.46 g/mol X Molarity X 1 ml = 400 mg X 0.085        Molarity = 0.094 MHydrocortisone molecular weight = 362.46 g/molmolarity (unknown) = mol/Lvolume = 1mlcomplex weight = 400 mgcomplex ratio = 85 mg Hydrocortisone/1 gm complex

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Reviews

1 of 2 reviewers received a sample product or took part in a promotion

Active Filters

  1. London, U.K.
    • Reviews 3
    • Votes 3
    5 out of 5 stars.

    Great supplement for endothelial cell culture

    Really good as a supplement to the M199 medium when wanting to culture primary human endothelial cells. As it is water soluble, you do not have to dissolve it in chloroform or ethanol like the other hydrocortisone products - it will dissolve directly into your medium of choice.

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  2. 1 Ratings-Only Review

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