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主要文件

HTK-

88022409, human bone, Not specified

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About This Item

分類程式碼代碼:
41106514
价格与库存信息目前不能提供

产品名称

HTK-, 88022409

生物源

human bone

增長模式

Adherent

染色體組型

Not specified

形態學

Not specified

產品

Not specified

受體

Not specified

技術

cell culture | mammalian: suitable

相關疾病

cancer

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Human osteomyeloma

細胞系描述

The cells are rendered TK- by selection with 50μg/ml BUdR. HTK- has the same STR profile as 143B (Sigma Catalogue number 91112502), both are TK-; it is likely that they are the same cell line by different names.

應用

Tumourigenicity studies

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18

培養基

EMEM (EBSS) + 2mM Glutamine + 25mg/l Bromo-deoxy Uridine (BUdR) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

其他說明

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