LLCMK2 (Original)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85062804, rhesus monkey kidney, Epithelial

• UNSPSC代码: 41106514

属性

• 生物来源: rhesus monkey kidney
• 描述: Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• 包装: tube of 5 μg 85062804-DNA-5UG; pkg of vial of cells 85062804-1VL
• 生长模式: Adherent
• 核型: 2n = 42
• 形态学: Epithelial
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Monkey Rhesus kidney

细胞系描述

Susceptible to a wide range of viruses including enterovirus, rhinovirus, myxovirus and poxvirus groups.

应用

LLCMK2 (Original) has been used to infect and maintain Trypanosoma cruzi parasite culture[1] and as a medium for Sendai virus titer determination.[2] It has also been used for viral culture.[3][4]

培养基

EMEM (EBSS) (M2279) + 2 mM L-Glutamine (G7513) + 1% NEAA (M7145) + 5% FBS / FCS (F2442).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37 °C.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.