Bu25 TK-

85022104, human cervix, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: Bu25 TK-, 85022104
• 生物源: human cervix
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human cervical carcinoma

細胞系描述

HeLa derivative rendered TK- by selection in BUdR.

應用

Transfection studies

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) seeding at 4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA. 5% CO₂; 37°C. Passage every 2-3 days when confluent at 1:3 dilution.

其他說明

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