IIC9 (CH0)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC代码: 41106514

属性

• 生物来源: hamster embryo
• 包装: tube of 5 μg 00030814-DNA-5UG; pkg of vial of cells 00030814-1VL
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Fibroblast
• 产品: Not specified
• 受体: PAR-1 (protease-activated receptor 1, thrombin receptor), EGF, PDGF
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Chinese hamster embryo fibroblast

细胞系描述

The Chinese hamster embryo fibroblast (CHEF) line is a nontumorigenic and stable diploid cell line, with a high serum requirement for growth. It is isolated from embryo preparation and is a spontaneous tumorigenic anchorage-independent line.[1][2]

应用

IIC9 (CH0) is suitable for selective growth of some rodent epithelial cells in a medium containing citrulline.[3]

培养基

DMEM:F12 (1:1) (D8062) + 5% FBS / FCS (F2442) + 2mM L-Glutamine (G7513).

传代培养常规

Split sub-confluent cultures (70-80%) 1:10 ie. seeding at 2-4 x10,000 cells/cm² using 0.25% trypsin /EDTA; 37°C; 5% CO₂.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.