推荐产品
生物来源
mouse
偶联物
unconjugated
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
AF3.1, monoclonal
表单
buffered aqueous solution
分子量
antigen 97 kDa
种属反应性
mouse, Xenopus, rat, bovine, canine, human
浓度
~2 mg/mL
技术
immunocytochemistry: suitable using 4% paraformaldehyde fixation, Triton X-100 permeabilization
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using a HeLa Cell nuclear extract
同位素/亚型
IgG2b
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... CDC27(996)
mouse ... Cdc27(217232)
rat ... Cdc27(360643)
一般描述
Monoclonal Anti-Cdc27 (mouse IgG2b isotype) is derived from the AF3.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to C-terminal of human Cdc27, conjugated to KLH.
The gene CDC27 (Cell division cycle protein 27 homolog) is mapped to human chromosome 17q12–23.2. The protein localizes in the nucleus.
免疫原
synthetic peptide corresponding to the C-terminus 10 residues of human Cdc27.
应用
Monoclonal Anti-Cdc27 antibody produced in mouse is suitable for the following applications:
- Immunocytochemistry using 4% paraformaldehyde fixation and Triton X-100 permeabilization
- Immunohistochemistry (formalin-fixed, paraffin-embedded sections)
- Immunoprecipitation.
- Indirect ELISA
- Microarray
- Western blotting / immunoblotting at a concentration of 1-2μg/mL using a HeLa Cell nuclear extract.
- Anaphase-promoting complex/cyclosome APC/C Purification.
生化/生理作用
CDC27 (Cell division cycle protein 27 homolog) is a component of anaphase-promoting complex/cyclosome (APC/C), an E3 ligase involved in cell cycle control. Phosphorylation of CDC27 is crucial for tumor growth factorβ (TGFβ)-induced activation of APC. It interacts with Microcephalin (MCPH1), causative gene for primary recessive autosomal microcephaly. DNA-damage response mediator MDC1 interacts with APC/C through CDC27. Down-regulation of CDC27 in irradiated cervical cancer cell line results in greater survival fraction. Polymorphism in CDC27 are linked with breast cancer.
The Cdc16/27/34 complex or the Anaphase-promoting complex/cyclosome (APC/C) complex functions as an ubiquitin conjugating enzyme, ubiquitinating cyclin B, and resulting in cyclin B/Cdk complex degradation.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Sanjay Shete et al.
Cancer research, 71(24), 7568-7575 (2011-11-01)
Gliomas, which generally have a poor prognosis, are the most common primary malignant brain tumors in adults. Recent genome-wide association studies have shown that inherited susceptibility plays a role in the development of glioma. Although first-degree relatives of patients exhibit
A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B
King R W, e al.
Cell, 81(2), 279-288 (1995)
Hui Guo et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 36(7), 5299-5304 (2015-02-15)
Cdc27, as a core component of anaphase-promoting complex (APC), is a cell cycle regulator, which participates in control of mitotic checkpoint and surveys the mitotic spindle to maintain chromosomal integrity. It was hypothesized that polymorphisms in cdc27 gene might contribute
T Rajkumar et al.
Indian journal of biochemistry & biophysics, 42(5), 271-278 (2005-10-01)
In the present study, an attempt was made to identify genes involved in radiation response in cervix carcinoma. Differential display technique was used to study the expression profiles of tumour biopsy samples obtained from patients, responding and not responding to
Gideon Coster et al.
The Journal of biological chemistry, 282(44), 32053-32064 (2007-09-11)
MDC1 (NFBD1), a mediator of the cellular response to DNA damage, plays an important role in checkpoint activation and DNA repair. Here we identified a cross-talk between the DNA damage response and cell cycle regulation. We discovered that MDC1 binds
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