This item is a product of PromoCell. The manufacturer considers the formulation proprietary. However, this is a complete medium that includes all the growth factors and supplements necessary for providing optimal cell health and efficient adherence selection of monocytes from freshly isolated mononuclear cells. This medium does not contain antibiotics or antimycotics and is formulated for use in an incubator with an atmosphere of 5% CO2.
价格与库存信息目前不能提供
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表单
aqueous solution
包装
pkg of 250 ml
技术
cell culture | mammalian: suitable
运输
wet ice
储存温度
2-8°C
一般描述
Monocyte Attachment Medium (Ready-to-use)
应用
The Monocyte Attachment Medium allows for the efficient adherence selection of monocytes from freshly isolated human mononuclear cells while maintaining optimal cell health. Thus, the time-consuming and costly immunomagnetic purification of monocytes is dispensable prior to experiments requiring adherent monocytes as a starting material. With properly performed washing steps large numbers of 80-90% pure monocytes can be obtained. Typical applications are generation of dendritic cells / macrophages as well as phagocytosis assays.Recommended for:Human mononuclear cells (freshly isolated)Human monocytes (freshly isolated)
质量
All lots of PromoCell Monocyte Attachment Medium are subjected to comprehensive quality control tests using primary human mononuclear cells from peripheral blood. Each lot of Monocyte Attachment Medium is checked for the efficient adherence selection of highly viable monocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
制备说明
The Medium is chemically defined/xeno-free and does not need further supplementation.
储存及稳定性
Store the Medium at 2 to 8°C in the dark immediately after arrival. Do not freeze the Medium. If stored properly, the product is stable until the expiry date stated on the label. After opening, the shelf life of the medium is 6 weeks at 2 to 8°C. For use, pre-warm only an aliquot of the medium and keep the remaining medium refrigerated at 2 to 8°C.
传代培养常规
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
商品
Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.
实验方案
Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.
使用无血清和无异源细胞培养基在10天内将人外周血单个核细胞(PBMC)体外分化为M1或M2巨噬细胞。欢迎探索我们超350种PromoCell产品。
Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.
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