This product may be reconstituted in sterile water at 0.1 - 1.0 mg/mL, following centrifugation of the vial to collect the contents. Please see the link below to review the product datasheet which includes complete preparation and storage instructions:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/399/841/g5035dat.pdf
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产品名称
粒细胞巨噬细胞集落刺激因子 人, GM-CSF, recombinant, expressed in E. coli, suitable for cell culture
生物来源
human
质量水平
重组
expressed in E. coli
方案
≥98% (SDS-PAGE)
表单
lyophilized powder
效能
≤0.1 ng/mL EC50
分子量
14.6 kDa
包装
pkg of 4X5 μg
pkg of 5 μg
储存条件
avoid repeated freeze/thaw cycles
技术
cell culture | mammalian: suitable
杂质
≤1.000 EU/μg
颜色
white
溶解性
water: soluble 0.010 mL, clear, colorless
UniProt登记号
储存温度
−20°C
基因信息
human ... CSF2(1437) , CSF2(1437)
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相关类别
一般描述
基于蛋白质含量的包装尺寸
生化/生理作用
粒细胞-巨噬细胞集落刺激因子(GM-CSF)是一种粒细胞、巨噬细胞和嗜酸性粒细胞谱系中的细胞生长和分化因子。GM-CSF在极低浓度下刺激多潜能祖细胞集落形成，是造血祖细胞在所有分裂过程中存活和增殖的重要因素。 它还能刺激一些上皮细胞和破骨细胞的生长。GM-CSF由多种细胞类型（单核细胞、内皮细胞、T细胞、成纤维细胞、丝裂原刺激的B细胞和脂多糖刺激的巨噬细胞）产生。GM-CSF是一种含有128个氨基酸的单链糖蛋白，采用保守二硫键。 人类和小鼠GM-CSF共享约。序列同源性54%，不发生生物活性交叉反应。
外形
从 0.2μm 过滤的 10mM 柠檬酸钠溶液 (pH3.5) 中冻干。
分析说明
使用人 TF-1 细胞在培养物中检测人 GM-CSF 的增殖活性。[1]
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
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Read article on hematopoietic cytokines and hematopoiesis
相关内容
Read article on hematopoietic cytokines and hematopoiesis
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What is reconstitution procedure for GM-CSF?
1 answer-
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Can I use Product G5035, Granulocyte-Macrophage Colony-Stimulating Factor human, on mouse cells?
1 answer-
There is only about 56% homology between human and mouse GM-CSF, and no cross-species activity. We would recommend to use mouse GM-CSF, Product No. G0282.
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There is no visible powder in the vial of Product G5035, Granulocyte-Macrophage Colony-Stimulating Factor human. Is the vial empty?
1 answer-
This product only contains 5 micrograms of protein without any carrier protein. The product is lyophilized from a small amount of solution so the product may not be visible in the vial.
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How many units are present in the vial of Product G5035, Granulocyte-Macrophage Colony-Stimulating Factor human?
1 answer-
The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is less than or equal to 0.1 ng/ml, corresponding to a specific activity of greater than or equal to 1 x 107 units/mg. So the 5 microgram package size (G5035-5UG) should have at least 50,000 units per vial.
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How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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What is the isoelectric point (pI) for Product G5035, Granulocyte-Macrophage Colony-Stimulating Factor human?
1 answer-
The pI is 4.8.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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