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Merck

C2306

Sigma-Aldrich

抗 兔 IgG(全分子)、F(ab′ 2 片段 –Cy3 绵羊抗

affinity isolated antibody, buffered aqueous solution

别名:

Anti-Rabbit IgG Cy3, Cy3 Anti-Rabbit IgG

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

sheep

共軛

CY3 conjugate

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

物種活性

rabbit

技術

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

一般說明

免疫球蛋白G(IgG)的单体结构由两条相同的重链和两条相同的轻链构成,其分子量分别为50kDa和25kDa。该抗体的一级结构还包含连接两条重链、连接重链和轻链,并位于链内的二硫键。IgG 可进一步分为四种亚型,即IgG1、IgG2、IgG3 和 IgG4,而这四种亚型所含的不同重链分别称为 γ1、 γ2、 γ3 和 γ4。使用木瓜蛋白酶的有限消化将抗体切割成三个片段,其中两个片段相同,并具有抗原结合活性(Fab片段)。
绵羊体内产生的抗兔 IgG(全分子)、F(ab′)2 片段 – Cy3 抗体可与所有兔 Ig 结合,在试图避免由于 Fc 受体的存在而导致背景染色时有用。

應用

绵羊抗兔IgG(全分子)、F(ab′)2片段-Cy3抗体可用于1:100替代的免疫组化和免疫细胞化学。

生化/生理作用

IgG抗体亚型是免疫系统中最丰富的血清免疫球蛋白。它由B细胞分泌,存在于血液和细胞外液中,可防止细菌、真菌和病毒引起的感染。母体IgG可通过胎盘转移至胎儿,胎盘对新生儿免疫防御感染至关重要。Cy3与抗兔IgG(全分子),F(ab′)2片断抗体的偶联可通过荧光显微镜观察蛋白质。

外觀

0.01 M 磷酸盐缓冲液 (pH 7.4) 中的溶液,含 1% 牛血清白蛋白和 15 mM 叠氮化钠

法律資訊

Cy由Amersham Biosciences Limited许可分销。

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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