推荐产品
應用
胶原酶已用于动脉组织的制备，用于晚期糖基化终产物 (AGE) 的研究。这种酶还与其他蛋白酶一起用于人类肿瘤、小鼠肾、人脑、肺上皮和许多其他组织的解聚。在肝脏和肾脏灌注研究、胰腺的消化和非实质肝细胞的分离中也有效。也适用于大鼠附睾脂肪垫脂肪细胞的制备。
生化／生理作用
有效地从组织中释放细胞需要胶原酶和中性蛋白酶的共同作用。每摩尔酶用 4 克原子钙 (Ca2+) 激活胶原酶。一般认为培养滤液至少含有 7 种不同的蛋白酶，分子量范围为 68-130 kDa（最适用 pH：6.3-8.8）。该酶识别序列-R-Pro-8-X-Gly-Pro-R-，其中 X 是最常见的中性氨基酸。
每摩尔胶原蛋白酶被4g原子钙活化。 它被乙二醇-双（β-氨基乙基醚）-N，NN′,N′-四乙酸，β-巯基乙醇、谷胱甘肽、巯基乙酸和8-羟基喹啉抑制。
注意
供应的产品可在-20°C 下可稳定保存一年。在 37°C、50°C 和-20°C 条件下储存 30 天，FALGPA 或蛋白酶活性无损失。快速冷冻 (10 mg/mL) 并在-20°C 下冷冻时，粗胶原酶溶液是稳定的。进一步冻融循环将破坏溶液。置于冰上 7 小时后，产品仍保持 100% 活性。
單位定義
一个胶原蛋白消化单元（CDU）从牛跟腱胶原蛋白中释放的肽链量和1.0 μ亮氨酸，5小时，pH7.4,37℃，在钙离子存在下的茚三酮颜色相同。一个 FALGPA水解单元每分钟，25℃，水解1.0 μmole 呋喃基丙烯酰-Leu-Gly-Pro-Ala。一个中性蛋白酶单元水解酪蛋白，产生颜色和1.0 μmole 酪氨酸每5小时，pH7.5,37℃下产生的相同。一个梭菌蛋白酶单元在DTT存在下，pH7.6,25℃，每分钟水解1.0 μmole BAEE。
準備報告
本品由溶组织梭菌的 Ⅱ 型 (C6885) 胶原酶制备而成。通常在 37°C 下用 TESCA 缓冲液（含 50 mM TES，0.36 mM 氯化钙，pH 7.4）制备 1-2 mg/mL 溶液。溶液还含有梭菌蛋白酶、非特异性中性蛋白酶和胰蛋白酶活性。
基底
訊號詞
Danger
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
其他客户在看
Neža Adamič et al.
Stem cell research & therapy, 13(1), 23-23 (2022-01-23)
Severe equine asthma (SEA) is a common chronic respiratory disease and a significant health and well-being problem in horses. Current therapeutic strategies improve pulmonary function and clinical signs in some horses, but in the long-term, return to full athletic function
Jessica M Kemppainen et al.
Biomaterials, 31(2), 279-287 (2009-10-13)
It has been widely postulated that scaffold permeability has a significant influence on chondrogenesis. However, since permeability has not been rigorously controlled in previous studies, there is no definitive conclusion as to how permeability affects cartilage regeneration by primary chondrocytes
Victoria Gudiño et al.
Cell death & disease, 12(10), 873-873 (2021-09-27)
RAC1B is a tumour-related alternative splice isoform of the small GTPase RAC1, found overexpressed in a large number of tumour types. Building evidence suggests it promotes tumour progression but compelling in vivo evidence, demonstrating a role in driving tumour invasion
Jeffrey Dock et al.
PloS one, 12(8), e0182498-e0182498 (2017-08-24)
A hallmark of human immunosenescence is the accumulation of late-differentiated memory CD8+ T cells with features of replicative senescence, such as inability to proliferate, absence of CD28 expression, shortened telomeres, loss of telomerase activity, enhanced activation, and increased secretion of
Marina G Yefimova et al.
Autophagy, 9(5), 653-666 (2013-02-27)
Phagocytosis and autophagy are typically dedicated to degradation of substrates of extrinsic and intrinsic origins respectively. Although overlaps between phagocytosis and autophagy were reported, the use of autophagy for ingested substrate degradation by nonprofessional phagocytes has not been described. Blood-separated
实验方案
To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.
在测定胶原酶活性时，使用N-（3-[2-呋喃基]丙烯酰基）-Leu-Gly-Pro-Ala在345nm处进行连续分光光度法测定。胶原酶水解胶原肽键。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门