推荐产品
生物源
bovine liver
品質等級
描述
optimum pH ~ 7.0
無菌
aseptically filled
形狀
aqueous solution
比活性
≥30,000 units/mg protein
分子量
tetramer ~250 kDa
包含
≤0.10 mg/mL Thymol
成份
ammonium sulphate, 30-50%
catalase, 10-20%
儲存條件
(Tightly closed. Keep locked up or in an area accessible only to qualified or authorized
persons)
技術
activity assay: suitable
等電點
5.4
pI
5.4
UniProt登錄號
運輸包裝
wet ice
儲存溫度
2-8°C
InChI
1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
InChI 密鑰
NUVBSKCKDOMJSU-UHFFFAOYSA-N
基因資訊
cow ... CAT(280743)
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一般說明
研究领域：细胞信号传导
来自牛的过氧化氢酶是一种四聚体酶，由四个相同的亚单位组成，每个亚单位含有一个血红素部分。每个子单元有四个域，如α-螺旋域，一个八链β-桶，通过缠绕环连接到β-桶的 N 端穿线臂。
来自牛的过氧化氢酶是一种四聚体酶，由四个相同的亚单位组成，每个亚单位含有一个血红素部分。每个子单元有四个域，如α-螺旋域，一个八链β-桶，通过缠绕环连接到β-桶的 N 端穿线臂。
Research Area: Cell Signaling
Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.
Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.
應用
来自牛肝的过氧化氢酶可用于：
- 在初级小鼠皮层神经元上进行单分子荧光原位杂交（smFISH）时，作为 GLOX 缓冲液的组分
- 进行与一氧化氮相互作用的结构和动力学分析
- 制备葡萄糖氧化酶过氧化氢酶体系（GOX-CAT 体系），以产生 H2O2，研究 H2O2 诱导的氧化应激对小鼠器官型小脑切片培养中髓鞘形成的影响
过氧化氢酶作为天然抗氧化剂，可用于研究活性氧物质对基因表达和凋亡的作用。它还被用于保护蛋白质、脂质和核酸免受氧化损伤。 工业上，过氧化氢酶已被用于去除添加至牛奶和奶酪中以及纺织品漂白使用的过氧化氢，并用于检查其对DNA修复大肠杆菌突变体活力的阳性效应。
生化／生理作用
过氧化氢酶是一种活跃的、普遍存在的酶，存在于所有需氧生物中。过氧化氢酶和氧化应激水平较低会导致几种疾病，如白癜风、高血压、脱氢酶血症、癌症、糖尿病和阿尔茨海默症。
Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT.
注意
过氧化氢酶溶液不可冷冻。冷冻溶液将导致酶活性损失50-70％。
單位定義
一单位酶可在pH7.0、25℃下每分钟分解1.0 μM的H2O2，通过测量A240的下降速率可知H2O2浓度从10.3降至9.2 mM。
準備報告
本品为含有超过30,000 单位/mg蛋白质的水溶液。为除去百里香酚防腐剂，可以通过离心使过氧化氢酶晶体沉淀，弃去上清液，将沉淀物重悬于水中，然后再次沉淀。该酶可溶于50 mM磷酸钾缓冲液，浓度为1 mg/mL，pH 7.0。
抑制劑
产品编号
说明
价格
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
其他客户在看
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Investigation of the binding mechanism and inhibition of bovine liver catalase by quercetin: Multi-spectroscopic and computational study
Samaneh R, et al.
BioImpacts, 7(3), 147?153-147?153 (2017)
Koodathingal Prakash et al.
Protein science : a publication of the Protein Society, 11(1), 46-57 (2001-12-14)
Catalases, although synthesized from single genes and built up from only one type of subunit, exist in heterogeneous form with respect to their conformations and association states in biological systems. This heterogeneity is not of genetic origin, but rather reflects
Namrta Purwar et al.
Biochemistry, 50(21), 4491-4503 (2011-04-29)
We present the structures of bovine catalase in its native form and complexed with ammonia and nitric oxide, obtained by X-ray crystallography. Using the NO generator 1-(N,N-diethylamino)diazen-1-ium-1,2-diolate, we were able to generate sufficiently high NO concentrations within the catalase crystals
实验方案
This procedure may be used for all Catalase products.
过氧化氢酶催化过氧化氢生成水和氧气。该实验方案使用分光光度法测定过氧化氢酶活性。
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