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Merck
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B1155

Sigma-Aldrich

双苯并咪唑 H 33258

powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC)

别名:

BBIH, BXI-72, HOE 33258, Hoechst 33258, NSC334072, 烟酸己可碱 三盐酸盐, 甲嗪双苯咪酚 三盐酸盐

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About This Item

经验公式（希尔记法）:
C25H24N6O · 3HCl
CAS号:
23491-45-4
分子量:
533.88
Beilstein:
4088183
EC號碼:
245-690-6
MDL號碼:
MFCD00012679
分類程式碼代碼:
41106305
PubChem物質ID:
24891565
NACRES:
NA.77

產品線

BioReagent

品質等級

200

化驗

≥98% (HPLC and TLC)

形狀

powder

技術

cell culture | mammalian: suitable

溶解度

H2O: 10 mg/mL
water and ethanol: 10 mg/mL

適合性

passes application test for fluorescence

儲存溫度

−20°C

SMILES 字串

Cl[H].Cl[H].Cl[H].[H]O[H].CN1CCN(CC1)c2ccc3NC(=NCc3c2)c4ccc5NC(=NCc5c4)c6ccc(O)cc6

InChI

1S/C27H28N6O.3ClH.H2O/c1-32-10-12-33(13-11-32)22-5-9-25-21(15-22)17-29-27(31-25)19-4-8-24-20(14-19)16-28-26(30-24)18-2-6-23(34)7-3-18;;;;/h2-9,14-15,34H,10-13,16-17H2,1H3,(H,28,30)(H,29,31);3*1H;1H2

InChI 密鑰

OWRSPPSBNWJJAR-UHFFFAOYSA-N

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應用

bisBenzimide H 33258可用于DNA、染色体和细胞核的染色。 bisBenzimide H 33258可用于荧光显微术或流式细胞术。
最大激发波长= 346nm
最大发射波长= 460nm

生化／生理作用

bisBenzimide H 33258是一种有效的选择性Bcl-XL抑制剂。 bisBenzimide H 33258是一种膜可渗透的荧光DNA染色剂，具有低细胞毒性，可嵌入DNA的A-T区域。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves

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Luke O'Shaughnessy et al.
Methods in molecular biology (Clifton, N.J.), 681, 295-309 (2010-10-28)
Expression of recombinant proteins in the baculovirus/insect cell expression system is employed because it enables post-translational protein modification and high yields of recombinant protein. The system is capable of facilitating the functional expression of many proteins - either secreted or
Maria Elena Dell'Aquila et al.
Fertility and sterility, 91(1), 249-255 (2008-03-28)
To analyze the effects of GnRH agonists versus antagonists on mitochondrial distribution and activity in human mature oocytes. Randomized research experimental study. Academic basic research laboratory and hospital-based fertility center. Two hundred twenty-five supernumerary mature oocytes from 44 patients. Fluorescent
Li Wang et al.
Frontiers in oncology, 11, 657008-657008 (2021-08-03)
Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive malignancy with extremely limited treatment; the effective targeting strategy stays an urgent unmet need. Neuropilin-2 (NRP2), a multifunctional transmembrane non-tyrosine-kinase glycoprotein, enhances various signal transduction pathways to modulate cancer progression. However, the
Luisa Valentini et al.
Animal reproduction science, 117(1-2), 166-177 (2009-04-18)
This study investigates the mitochondrial (mt) distribution in canine ovarian oocytes examined at recovery time, as related to the reproductive cycle stage, and in oviductal oocytes. Ovarian Germinal Vesicle (GV) stage oocytes were recovered from bitches in anestrous (A, n=2)
Jared J Luxton et al.
Cell reports, 33(10), 108457-108457 (2020-11-27)
Telomere length dynamics and DNA damage responses were assessed before, during, and after one-year or shorter duration missions aboard the International Space Station (ISS) in a comparatively large cohort of astronauts (n = 11). Although generally healthy individuals, astronauts tended to
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