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MP0025

Sigma-Aldrich

Venor™ GeM 支原体检测试剂盒，基于 PCR

别名:

PCR mycoplasma detection kit

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

用途

 kit sufficient for 25 tests

品質等級

200

包裝

pkg of 1 kit

儲存條件

dry at room temperature

技術

PCR: suitable

應用

detection
microbiology

儲存溫度

2-8°C

相关类别

一般說明

Venor™ GeM 支原体检测试剂盒利用聚合酶链反应（PCR），成为检测细胞培养物和其它细胞培养衍生生物制品中支原体和无胆甾原体污染的灵敏度最高的首选方法。检测需要低至 1-5fg 的支原体 DNA，对应于每个样品 2-5 个支原体。引物组对高度保守的 rRNA 操纵子有特异性，更具体而言，对支原体基因组中的 16S rRNA 编码区有特异性。这让我们可以检测迄今为止测试的所有支原体、无胆甾原体和脲原体物种，这些物种通常是细胞培养物中的污染物。该试剂盒不可扩增真核和细菌 DNA。

應用

基于 PCR 的 Venor™ GeM 支原体检测试剂盒采用 PCR 技术快速可靠地检测细胞培养物和病毒原液中的支原体 DNA。

成分

不包括Taq聚合酶。 优化用于 D9307、Taq DNA 聚合酶

儲存和穩定性

该试剂盒在环境温度下运输时是稳定的。收货后，在 2–8 °C下储存。 引物/核苷酸混合物、阳性对照和内对照再水化后，在 –20 °C 以下储存，避免反复冻融。对于低样品数的重复测试，引物/核苷酸混合物和对照应在再水化后等分。遵循这些建议，该试剂盒在标签上注明的失效日期之前是稳定的。

法律資訊

Venor is a trademark of Minerva Biolabs GmbH

仅试剂盒组分

产品编号
说明
  • Positive Control
  • Negative Control
  • PCR 10X Reaction Buffer 500 mL/vial
  • Primer/Nucleotide Mix 1 mL/vial

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Mycoplasma detection in a historical arbovirus repository: Commercial kit comparison and implications for improved repository management
Russell BJ, et al.
Journal of Virological Methods (2020)
Evolution of Hoxa11 regulation in vertebrates is linked to the pentadactyl state
Kherdjemil Y, et al.
Nature (2016)
Julie V Philley et al.
Journal of cellular physiology, 231(6), 1364-1374 (2015-11-05)
Mitochondria (mt) encoded respiratory complex-I (RCI) mutations and their pathogenicity remain largely unknown in prostate cancer (PCa). Little is known about the role of mtDNA loss on mt integrity in PCa. We determined mtDNA mutation in human and mice PCa
Tomohiro Aoki et al.
Cancer discovery, 10(3), 406-421 (2019-12-21)
Hodgkin lymphoma is characterized by an extensively dominant tumor microenvironment (TME) composed of different types of noncancerous immune cells with rare malignant cells. Characterization of the cellular components and their spatial relationship is crucial to understanding cross-talk and therapeutic targeting
Anbarasu Kannan et al.
Scientific reports, 7, 46102-46102 (2017-04-07)
Human papilloma virus-16 (HPV-16) associated oropharyngeal cancer (HPVOPC) is increasing alarmingly in the United States. We performed whole genome sequencing of a 44 year old, male HPVOPC subject diagnosed with moderately differentiated tonsillar carcinoma. We identified new somatic mutation in
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