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Merck

A9228

Sigma-Aldrich

淀粉葡萄糖苷酶 来源于根霉菌

≥40,000 units/g solid

别名:

1,4-α-D-葡聚糖葡萄糖水解酶, 糖化淀粉酶, 葡萄糖淀粉酶

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

$1,300.00


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表单

lyophilized solid

质量水平

比活

≥40,000 units/g solid

溶解性

0.03 M sodium citrate-phosphate buffer, pH 4.5: soluble 5.0 mg/mL

储存温度

−20°C

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应用

淀粉葡萄糖苷酶用于水解 α-D-葡糖苷。 它可用于啤酒的酿造和面包和果汁的生产。 来自根霉菌属的淀粉葡萄糖苷酶,已被用于研究在 ER 相关的蛋白质降解(ERAD)期间寡糖的裂解。[1]该酶已用于 N-香草基 - 壬酰胺的糖基化,形成具有药理学应用的水溶性组份。[2]灰盖鬼伞菌的菌柄残留物为材料,用淀粉葡糖苷酶处理,测定其糖原含量。[3]

生化/生理作用

淀粉葡萄糖苷酶能够水解寡糖的 α-D-(1-4),α-D-(1-6)和 α-D-(1-3)糖苷键[4]

单位定义

一个单位将在 pH 4.5,55℃ 下,在 3 分钟内,从淀粉中释放 1.0mg 的葡萄糖。

外形

冻干的无盐粉末

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Isabelle Chantret et al.
The Journal of biological chemistry, 286(48), 41786-41800 (2011-10-08)
In Saccharomyces cerevisiae, proteins with misfolded lumenal, membrane, and cytoplasmic domains are cleared from the endoplasmic reticulum (ER) by ER-associated degradation (ERAD)-L, -M, and -C, respectively. ERAD-L is N-glycan-dependent and is characterized by ER mannosidase (Mns1p) and ER mannosidase-like protein
Ramaiah Sivakumar et al.
Biotechnology letters, 29(10), 1537-1548 (2007-07-05)
Enzymatic syntheses of N-vanillyl-nonanamide, 1, glycosides with D-glucose, 2, D-galactose, 3, D-mannose, 4, D-ribose, 5, maltose, 6, and lactose, 7, were carried out using amyloglucosidase from Rhizopus and beta-glucosidase from sweet almond. The latter catalysed the syntheses of N-vanillyl-nonanamide glycosides
Daisuke Sugiura et al.
Plant & cell physiology, 58(12), 2043-2056 (2017-12-08)
To clarify whether excessive accumulation of total non-structural carbohydrate (TNC) causes down-regulation of photosynthesis in Raphanus sativus, we manipulated sink-source balance to alter TNC levels in source leaves and examined its effects on photosynthetic characteristics, whole-plant biomass allocation and anatomical
Masabumi Komatsu et al.
Physiologia plantarum, 147(2), 159-168 (2012-05-23)
To determine the effects of elevated CO(2) concentration ([CO(2)]) on the temperature-dependent photosynthetic properties, we measured gas exchange and chlorophyll fluorescence at various leaf temperatures (15, 20, 25, 30, 35 and 40°C) in 1-year-old seedlings of the Japanese white birch
Daisuke Sugiura et al.
Annals of botany, 123(4), 715-726 (2018-12-06)
Sink-source imbalance could cause an accumulation of total non-structural carbohydrates (TNC; soluble sugar and starch) in source leaves. We aimed to clarify interspecific differences in how sink-source imbalance and TNC causes the downregulation of photosynthesis among three legume plants. The

实验方案

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

本操作程序可用于使用淀粉作为底物测定淀粉葡萄糖苷酶的活性。

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