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A8181

Sigma-Aldrich

氨基黑染色液 2 X

electrophoresis reagent

别名:

萘酚蓝黑 溶液, 氨基黑 10B

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About This Item

经验公式（希尔记法）:
C22H14N6Na2O9S2
CAS号:
1064-48-8
分子量:
616.49
Beilstein:
5374263
MDL號碼:
MFCD00004017
分類程式碼代碼:
12171500
PubChem物質ID:
24891210
NACRES:
NA.32

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品質等級

200

形狀

liquid

SMILES 字串

[Na+].[Na+].Nc1c(\N=N/c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\C=C/c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;

InChI 密鑰

AOMZHDJXSYHPKS-DROYEMJCSA-L

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相关类别

一般說明

氨基黑(AB)是一种用于蛋白染色的酸性染料，[1]可检测聚偏二氟乙烯(PVDF)和硝酸纤维素膜中50ng级的蛋白水平。[2]氨基黑对于低浓度蛋白也能显色。[3]因其可对指纹中的血液蛋白进行染色，适于法医学应用。[1]

應用

氨基黑染色液2X已用于染色：
  • 培养上清液中的血清蛋白[4]
  • 小鼠肝脏匀浆中的半胱氨酸亚磺酸脱羧酶[5]
  • 染色质样品的组蛋白[6]

重構

用去离子水稀释至终体积 500 后ml，溶液含 0.1%(w/v) 氨基黑，25%(v/v) 异丙醇和 10%(v/v) 乙酸。

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产品编号
说明
价格

象形圖

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GHS02,GHS07

訊號詞

Danger

危險分類

Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3

標靶器官

Central nervous system

儲存類別代碼

3 - Flammable liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

53.6 °F

閃點（°C）

12 °C

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCP7209
SLCK9090
SLCF5171
SLBW0939
SLBR9882V

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Protein Blotting and detection: methods and protocols, 86(1), 10-18 (2009)
Meghan C Hughes et al.
The Journal of physiology, 598(7), 1377-1392 (2019-01-24)
Ninety-eight per cent of patients with Duchenne muscular dystrophy (DMD) develop cardiomyopathy, with 40% developing heart failure. While increased propensity for mitochondrial induction of cell death has been observed in left ventricle, it remains unknown whether this is linked to
Detection of proteins on blot membranes
Goldman A, et al.
Current Protocols in Protein Science / Editorial Board, John E. Coligan ... [Et Al.], 86(1), 10-18 (2016)
Placental release of taurine to both the maternal and fetal circulations in human term pregnancies
Holm MB, et al.
Amino Acids, 50(9), 1205-1214 (2018)
Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a positively charged membrane filter.
J M Gershoni et al.
Analytical biochemistry, 124(2), 396-405 (1982-08-01)
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蛋白电泳染色剂选择指南

为满足蛋白分析的多样化需求，Sigma提供多种蛋白质可视化（染色）试剂。EZBlue™和ProteoSilver™专为蛋白质组学而设计，即使在传统的PAGE形式中依然表现出色。

Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Questions

  1. Could you please provide the staining procedure and destaining formulation for the Amino Black Staining solution 2x that is suitable for staining in 2-40% gels?

    1 answer

    1. To detect total protein on a Western blot, follow these steps:
      1.Prepare Amido Black 1X Staining Solution by diluting Amido Black Staining Solution (Product Number A8181) 1:1 with deionized water.
      2. After transferring proteins onto nitrocellulose membrane, immerse membrane in sufficient Amido Black 1X Staining Solution to cover. Stain for 1 minute.
      3. Destain the membrane by placing in an aqueous solution containing 25% isopropanol and 10% acetic acid for 30 minutes. If a lighter background is desired, destain overnight.
      4. Store the destained membrane in deionized water or allow to air dry.
      Product Number A8181 Store at Room Temperature Product Description: Amido Black staining solution is used for the detection of microgram quantities of proteins transferred to nitrocellulose membranes. In comparison to staining with Brilliant Blue R, staining with Amido Black results in significantly less background on nitrocellulose membranes. Because nitrocellulose membranes are more stable in isopropanol than in methanol or other solvents, the formulation contains isopropanol. After a 1:1 dilution with deionized water, Amido Black Staining Solution contains: 0.1% Amido Black (w/v), 25% Isopropanol (v/v), 10% Acetic Acid (v/v). Note: Not suitable for use with charge modified nylon membranes.

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