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Merck

A3937

Sigma-Aldrich

抗-兔IgG(全分子),F(ab′)2 片段−碱性磷酸酶 山羊抗

affinity isolated antibody, buffered aqueous glycerol solution

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

goat

共軛

alkaline phosphatase conjugate

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous glycerol solution

物種活性

rabbit

技術

direct ELISA: 1:30,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

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一般說明

IgG是糖蛋白抗体,可调控几种免疫应答。靶蛋白的兔IgG通常在多种研究应用中用作一抗。因此,与可检测底物缀合的抗兔IgG二抗是靶蛋白分析的有用工具。抗兔IgG(全分子),(F(ab′)2)片段-碱性磷酸酶抗体结合全部兔免疫球蛋白。

免疫原

纯化兔 IgG

應用

以碱性磷酸酶标记的山羊抗兔F2片段特异性抗体为二抗,在室温下对前列腺上皮细胞裂解液(1:7500稀释)进行2小时蛋白质印迹分析。
成功使用该抗体的应用以及相关同行评审论文如下所示。
酶联免疫吸附试验免疫印迹法(1 篇)
蛋白印迹法(1 篇)
抗兔IgG(全分子),(F(ab′)2)片段-碱性磷酸酶抗体适用于免疫印迹(5μL)和直接ELISA(1:30,000)。该产品也可用于免疫组织化学(1:50,使用福尔马林进行固定、石蜡包埋切片)。

外觀

溶剂为0.05 M Tris缓冲液,pH 8.0,含1 mM MgCl2、10 mM甘氨酸、1%牛血清白蛋白、50%甘油和15 mM叠氮化钠

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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The basis for the unusual properties of the catalytic subunit (C) of ram sperm cAMP-dependent protein kinase was investigated. Ram sperm C was purified and found by mass spectrometry (MS) to be approximately 890 Da smaller than Calpha, the predominant
Stromal and Epithelial Expression of Tumor Markers Hyaluronic Acid and HYAL1 Hyaluronidase in Prostate Cancer
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Journal of Biochemistry, 276, 11922-11932 (2001)
Naoya Takanashi et al.
BMC microbiology, 13, 54-54 (2013-03-19)
Previously, a bovine intestinal epithelial cell line (BIE cells) was successfully established. This work hypothesized that BIE cells are useful in vitro model system for the study of interactions of microbial- or pathogen-associated molecular patterns (MAMPs or PAMPs) with bovine
Keiko Igaki et al.
International immunopharmacology, 60, 160-169 (2018-05-08)
C-C chemokine receptor 9 (CCR9) is the homing receptor for C-C motif chemokine ligand 25 (CCL25), and contributes to the maintenance of mucosal immunity and pathogenesis of inflammatory bowel disease (IBD) through the recruitment of T cells into the gut
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Circulation research, 111(4), 415-425 (2012-06-21)
Viral myocarditis results from an adverse immune response to cardiotropic viruses, which causes irreversible myocyte destruction and heart failure in previously healthy people. The involvement of microRNAs and their usefulness as therapeutic targets in this process are unknown. To identify

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