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一般說明
凝胶上样溶液在电泳期间可用作跟踪染料。染料具有轻微的负电荷，并且与DNA的迁移方向相同，从而使得用户可以监测分子在凝胶中移动的进程。迁移速率随凝胶组成而变化。上样前用样品进行1：6稀释。
應用
适用于琼脂糖或非变性聚丙烯酰胺凝胶电泳（PAGE），该电泳步骤可能是Northern和Southern印迹杂交过程的一部分。
成分
凝胶上样缓冲液含有0.25％溴酚蓝、0.25％二甲苯蓝和40％蔗糖。
其他說明
预期条带迁移如下：
在聚丙烯酰胺凝胶上，二甲苯氰基可与大约450-460bp的DNA共迁移，而溴酚蓝与15-100bp的DNA共迁移。在0.5-1.4％琼脂糖凝胶上，二甲苯氰基可与4kb dsDNA共迁移，而溴酚蓝则与300bp dsDNA共迁移。
在聚丙烯酰胺凝胶上，二甲苯氰基可与大约450-460bp的DNA共迁移，而溴酚蓝与15-100bp的DNA共迁移。在0.5-1.4％琼脂糖凝胶上，二甲苯氰基可与4kb dsDNA共迁移，而溴酚蓝则与300bp dsDNA共迁移。
相關產品
产品编号
说明
价格
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
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We have characterized an early series of 5,6-bridged dioxinoquinolones which behaved strikingly different from typical quinolones. The 5,6-bridged dioxinoquinolones inhibited Escherichia coli DNA gyrase supercoiling activity but, unlike typical quinolones, failed to stimulate gyrase-dependent cleavable complex formation. Analogous unsubstituted compounds
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