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Merck
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文件

G7654

Sigma-Aldrich

凝胶上样液

for NA electrophoresis, solution

别名:

DNA Gel Loading Solution, Gel Loading Buffer

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About This Item

分類程式碼代碼:
12161703
NACRES:
NA.25

一般說明

凝胶上样溶液在电泳期间可用作跟踪染料。染料具有轻微的负电荷，并且与DNA的迁移方向相同，从而使得用户可以监测分子在凝胶中移动的进程。迁移速率随凝胶组成而变化。上样前用样品进行1：6稀释。

應用

适用于琼脂糖或非变性聚丙烯酰胺凝胶电泳（PAGE），该电泳步骤可能是Northern和Southern印迹杂交过程的一部分。

成分

凝胶上样缓冲液含有0.25％溴酚蓝、0.25％二甲苯蓝和40％蔗糖。

其他說明

预期条带迁移如下：
在聚丙烯酰胺凝胶上，二甲苯氰基可与大约450-460bp的DNA共迁移，而溴酚蓝与15-100bp的DNA共迁移。在0.5-1.4％琼脂糖凝胶上，二甲苯氰基可与4kb dsDNA共迁移，而溴酚蓝则与300bp dsDNA共迁移。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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