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D5796

Sigma-Aldrich

DMEM - high glucose

Dulbecco’s Modified Eagle’s Medium - high glucose

With sodium Bicarbonate and ʟ-glutamine, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

别名:

DME, DMEM

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500 ML
$83.60
1 L
$128.00
6 X 500 ML
$433.00

$83.60

国内现货，预计发货时间2025年4月07日详情

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选择尺寸

变更视图
500 ML
$83.60
1 L
$128.00
6 X 500 ML
$433.00

About This Item

UNSPSC代码:
12352207
NACRES:
NA.75

$83.60

国内现货，预计发货时间2025年4月07日详情

获取大包装报价

产品名称

杜氏改良 Eagle 培养基 - 高葡萄糖, With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

无菌性

sterile-filtered

表单

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

NaHCO3: yes
L-glutamine: yes
glucose: high
phenol red: yes
sodium pyruvate: no
HEPES: no

运输

ambient

储存温度

2-8°C

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一般描述

这种DMEM高葡萄糖培养基是一种不含添加因子（常见修改）如L-丙氨酰-L-谷氨酰胺、HEPES或丙酮酸钠的1x完全培养基。 它与原始DMEM高葡萄糖配方的不同点在于用吡哆醇代替了吡哆醛。 吡哆醛是一种不稳定的培养基成分。

应用

Dulbecco′s Modified Eagle′s Medium（DMEM）是对Basal Medium Eagle (BME)的一种改良，含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此，它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养，以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外，葡萄糖浓度也被提高至4500 mg/L，因此被命名为“DMEM/High”。

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000416297
0000416297
0000375761
0000373457
0000375762

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Questions

1–3 of 3 Questions  

  1. What are the product numbers for DMEM and EMEM used to grow the 293 and 293T cell lines?

    1 answer

    1. To grow the 293 and 293T cells, the following product recommendations are provided:

      For the 293 cell line (product number 85120602), ECACC recommends using EMEM media (EBSS) with 2mM Glutamine, 1% Non Essential Amino Acids (NEAA), and 10% FCS. ECACC uses the product number M4655 for this purpose.

      For the 293T cell line (product number 12022001), ECACC recommends using DMEM media with 2mM Glutamine and 10% Fetal Bovine Serum (FBS). ECACC uses the product number D5796 media for sub-culturing this cell line.

      Helpful?

  2. What is the conc. of L glutamine

    1 answer

    1. The concentration of L-Glutamine in this product is 0.584 g/L. Please see the link below to review the DMEM Formulation Tables: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/340/109/d5796for.pdf

      Helpful?

  3. pH는 몇으로 조정되어 있나요?

    1 answer

    1. The pH specification of this product is 7.0-7.6. The exact value will be reported in the lot specific Certificate of Analysis. Please see the link below to review a sample Certificate of Analysis: https://www.sigmaaldrich.com/coa/SIGMA/D5796/RNBL9771.

      Helpful?

Reviews

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  1. Columbia, MO
    • Reviews 2
    • Vote 1
    5 out of 5 stars.

    DMEM

    I always feel confident when using Sigma cell culture media.

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  2. 2 Ratings-Only Reviews

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