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Merck

D8437

Sigma-Aldrich

DMEM/F-12

With ʟ-glutamine, HEPES, sodium pyruvate and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

别名:

DME/F-12, 1:1混合

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

product name

杜氏改良Eagle 培养基/营养混合物 F-12 Ham, With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

品質等級

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

成分

glucose: 3.15 g/L
HEPES: yes
L-glutamine: yes
phenol red: yes
sodium pyruvate: 0.055 g/L

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

杜氏改良Eagle培养基(DMEM)是一种富含能量的常用细胞培养基,包含葡萄糖、维生素和氨基酸。杜氏改良Eagle培养基(DMEM)与Ham F-12的1:1营养混合物常用于细胞培养研究。为了弥补去除血清造成的缓冲能力损失,可以在配方中以15 mM的最终浓度添加4-(2-羟乙基)-1-哌嗪乙磺酸(HEPES)缓冲液。

應用

杜氏改良Eagle培养基/营养混合物F-12 Ham:
  • 已用作培养基的组分,以培养原代培养激活的人肝星状细胞
  • 已用于培养软骨切片
  • 可用于考德里氏体的繁殖

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Nutrient Mixture F-12 Ham Formulation

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