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Merck
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D7777

Sigma-Aldrich

杜氏改良 Eagle 培养基 - 高葡萄糖

With 4500 mg/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture

别名:

DME, DMEM

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

品質等級

500

形狀

powder

技術

cell culture | mammalian: suitable

成分

glucose: high
sodium pyruvate: yes
NaHCO3: no
HEPES: no
phenol red: yes
L-glutamine: yes

運輸包裝

ambient

儲存溫度

2-8°C

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應用

Dulbecco′s Modified Eagle′s Medium（DMEM）是对Basal Medium Eagle (BME)的一种改良，含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此，它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养，以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外，葡萄糖浓度也被提高至4500 mg/L，因此被命名为“DMEM/High”。

數量

按照每升培养基13.5克粉末配制。

重構

补充 3.7g/L 碳酸氢钠

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儲存類別代碼

13 - Non Combustible Solids

水污染物質分類（WGK）

WGK 1

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实验方案

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

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