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方案
≥80.0% (degree of coupling)
表单
solid
溶解性
DMF: 1 mg/mL, clear
荧光
λex 480 nm; λem 610-630 nm in PBS, pH 7.4
储存温度
−20°C
一般描述
Abberior STAR 470SX is the latest development of long-Stokes-Shift dyes for STED microscopy. The dye can be excited from 450 to 480nm. It can substitute dyes like Chromeo™ 494. For STED, a depletion wavelength ~750 nm is recommended. It is therefore well suited for 2-color STED imaging as implemented in the Leica TCS STED Ti:Sa microscope.
Abberior STAR 470SX is the dye of choice for long Stokes STED applications in the orange fluorescent regime. The dye is particularly designed and tested for 2-color STED microscopy in combination with our STAR 635 using a single STED wavelength. The dye is our recommendation for usage in the Leica TCS STED Ti:Sa 2-color system.
Key Features
Absorption Maximum, λmax: 475 nm (MeOH),
477 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 30′400 M-1cm-1 (MeOH),
22′700 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.69
Correction Factor, CF280 = ε280/max: 0.47
Fluorescence Maximum, λfl: 609 nm (MeOH),
627 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 740 - 770 nm
Fluorescence Quantum Yield, η: 0.80 (EtOH)
Fluorescence Lifetime, τ: 3.9 (EtOH)
Abberior STAR 470SX is the dye of choice for long Stokes STED applications in the orange fluorescent regime. The dye is particularly designed and tested for 2-color STED microscopy in combination with our STAR 635 using a single STED wavelength. The dye is our recommendation for usage in the Leica TCS STED Ti:Sa 2-color system.
Key Features
- Designed for STED microscopy at ~750 nm
- Long Stokes′ shift (>130 nm) for 2-color applications
- Tested in the Leica TCS STED Ti:Sa 2-color system
Absorption Maximum, λmax: 475 nm (MeOH),
477 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 30′400 M-1cm-1 (MeOH),
22′700 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.69
Correction Factor, CF280 = ε280/max: 0.47
Fluorescence Maximum, λfl: 609 nm (MeOH),
627 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 740 - 770 nm
Fluorescence Quantum Yield, η: 0.80 (EtOH)
Fluorescence Lifetime, τ: 3.9 (EtOH)
应用
Abberior® STAR 470SX goat anti-rabbit antibody has been used for STED (stimulated emission depletion) microscopy in Caco-2 cells.
适用性
Designed and tested for fluorescent super-resolution microscopy
法律信息
Chromeo is a trademark of Active Motif Chromeon GmbH
abberior is a registered trademark of Abberior GmbH
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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