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Merck

88417

Sigma-Aldrich

MTT 甲臜

MTT Formazan

MTT reduction end product, powder

别名:

1-(4,5-二甲基吡啶-2-基)-3,5-二苯基甲酰胺, 噻唑基蓝甲臜

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250 MG
$76.50
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$258.00

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250 MG
$76.50
1 G
$258.00

About This Item

经验公式(希尔记法):
C18H17N5S
CAS号:
分子量:
335.43
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.83

$76.50


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产品名称

MTT 甲臜, MTT reduction end product

表单

powder

溶解性

chloroform: 10 mg/mL, clear, dark violet

SMILES字符串

Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C

InChI

1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+

InChI key

GIFKVQOPAJDJLO-SZVLODPRSA-N

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应用

MTT甲臜用于细胞活率检测。[1][2][3]

生化/生理作用

MTT法是一种比色反应分析方法,常用于检测细胞活率。该方法要求将溶于水的黄色3-[4,5-二甲基噻唑-2-yl]-2,5-基四氮唑嗅盐(MTT)酶促还原为不溶于水的紫色MTT-甲臜。该反应由线粒体脱氢酶催化,仅在活细胞中发生。[4][5]因此,蓝色甲臜量与活细胞数量成正比。通过分光光度法分析570 nm波长处的甲臜溶液吸光度。[6]

其他说明

评审:四唑盐和甲瓒[7]

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

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Questions

Reviews

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    • Review 1
    • Votes 8
    4 out of 5 stars.

    Will Not Work For MTT Proliferation Assay

    I accidentally bought this thinking it was MTT salt to use in a cell proliferation assay but it is MTT Formazan, the water insoluble end product! Therefore, it is useless for the assay. I suppose you could use it as a control to optimize your buffer for solubilizing the insoluble Formazan. The description for this product is misleading and should clarify that this is not what you need for an MTT assay, and refer the customer to MTT salt. That said, this is a nice purple crystalline powder and insoluble in water consistent with MTT Formazan, if that is what you actually want.

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