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M2128

Sigma-Aldrich

噻唑蓝

98%, powder

别名:

3-(4,5-二甲基-2-噻唑)-2,5-二苯基溴化四氮唑噻唑蓝, 噻唑四唑蓝溴化盐, 噻唑蓝四唑蓝

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100 MG
$77.30
250 MG
$126.00
500 MG
$231.00
1 G
$384.00
5 G
$1,450.00
10 G
$2,610.00

$77.30

国内现货，预计发货时间2025年4月07日详情

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选择尺寸

变更视图
100 MG
$77.30
250 MG
$126.00
500 MG
$231.00
1 G
$384.00
5 G
$1,450.00
10 G
$2,610.00

About This Item

经验公式（希尔记法）:
C18H16BrN5S
CAS号:
298-93-1
分子量:
414.32
Beilstein:
4081397
EC 号:
206-069-5
MDL编号:
MFCD00011964
UNSPSC代码:
12352111
PubChem化学物质编号:
24896603
NACRES:
NA.47

$77.30

国内现货，预计发货时间2025年4月07日详情

获取大包装报价

产品名称

噻唑蓝, 98%

方案

98%

表单

powder

技术

titration: suitable

颜色

dark yellow

mp

195 °C (dec.) (lit.)

溶解性

2-methoxyethanol: soluble 20 mg/mL
ethanol: soluble 20 mg/mL
H2O: 5 mg/mL

应用

diagnostic assay manufacturing
hematology
histology

储存温度

2-8°C

SMILES字符串

[Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4

InChI

1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1

InChI key

AZKSAVLVSZKNRD-UHFFFAOYSA-M

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应用

噻唑蓝四唑溴化物已用于以下检测：
  • 增殖检测。[1][2]
  • TNF（肿瘤坏死因子）生物检测。[3]
噻唑蓝四唑蓝(MTT)可用于细胞增殖的检测。 MTT产生一种淡黄色的溶液，通过活细胞的线粒体脱氢酶将其转化为深蓝色、不溶于水的MTT福尔马赞。该蓝色晶体可溶于酸化异丙醇，可采用比色法测定其570 nm处的强度。
噻唑蓝四唑蓝(MTT)可用于细胞增殖的检测。 MTT产生一种淡黄色的溶液，通过活细胞的线粒体脱氢酶将其转化为深蓝色、不溶于水的MTT福尔马赞。该蓝色晶体可溶于酸化异丙醇，可采用比色法测定其570 nm处的强度。MTT已被用作组织化学/细胞化学试剂 以及用于检测NAD。 由于所使用的氰化物陷阱可与阳离子结合，组织中的ADP-连接酶系统无法采用MTT检测。 MTT迅速还原为福尔马赞，福尔马赞与镍、铜和钴螯合;钴螯合物已可用于氧化体系。

象形图

Health hazardExclamation mark
GHS08,GHS07

警示用语：

Warning

危险分类

Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCV1532
MKCR0748
MKCR7905
SHBN4138
MKCL9866

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XTT 钠盐 powder, BioReagent, suitable for cell culture

Sigma-Aldrich

X4626

XTT 钠盐

细胞增殖试剂盒II（XTT） liquid, pkg of 1 kit, suitable for cell analysis, suitable for tissue culture

Roche

11465015001

细胞增殖试剂盒II（XTT）

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Chang WH, et al.
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实验方案

Recycling Micro-Assay of β-NAD and β-NADH

Objective: To standardize a procedure for the recycling micro-assay of β-NAD and β-NADH

β-NAD和β-NADH的回收微量分析

在进行β-NAD和β-NADH的回收微量分析时，使用了在570 nm处的连续分光光度法进行测定。

Questions

1–10 of 10 Questions  

  1. I used 0.05g in 10ml then syringe it and add 40ml. My question is how to calculate the percentage of viable cells? I used DMSO after MTT solution

    1 answer

    1. MTT is typically used for the semi-quantitative colorimetric measurement of cell viability. This analysis is performed in a multi-well format which screens for the viability of any given compound at any given concentration. The percentage of viable cells cannot be determined using this method. After adding a stop reagent, in this case DMSO, the formazan crystals will be dissolved, making manual counting a challenge, at best. When used in a multi-well plate format, cells are incubated, a stop solution is added, and the plate is read spectrophotometrically at 570 nM. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/305/017/m2128pis.pdf

      For additional information, see the link below to the MTT kit protocol for product TOX1, a cytotoxicity kit that utilized MTT:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/177/tox1bul.pdf

      To measure cell viability as a percentage of an entire population, microscopic methods may be of interest. Reagents such as Trypan Blue allow for the clear identification of viable and non-viable cells. To review more information regarding this method, please see the link below:
      https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/cell-quantification

      Helpful?

  2. What is the recommendation volume of 5 mg/ml of thiazolyl blue tetrazolium blue (in uL) per well to use for MTT assay? 

    1 answer

    1. Instructions for use are not provided for this product. However, there is an in vitro toxicology assay kit MTT based available. The catalog number is TOX1-1KT. In the kit, 15 mg of solid MTT is supplied. This is resuspended with 3 ml of media or a balanced salt solution without phenol red or serum. The link shared below will list the complete procedure

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/177/tox1bul.pdf

      Helpful?

  3. What can be used to solubilize MTT formazan (the product of the Thiazolyl Blue Tetrazolium Bromide - MTT reaction)?

    1 answer

    1. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring of the MTT, yielding purple MTT formazan crystals. These crystals are not soluble in aqueous solutions; they may be dissolved in acidified isopropanol (0.1 N HCl in anhydrous isopropanol). If this does not lyse the cells, Triton X-100 can be added to the acidified isopropanol. A typical working concentration is 10% Triton X-100 (i.e., 1 volume of 100% Triton X-100 + 9 volumes of acidified isopropanol).

      Helpful?

  4. How can Thiazolyl Blue Tetrazolium Bromide be used for staining dehydrogenase in tissue sections?

    1 answer

    1. Products M2128 or M5655, Thiazolyl Blue Tetrazolium Bromide can be used for staining dehydrogenase in tissue sections. See the method below. Reagent PreparationMTT (2 mg/ml distilled water) 2.5 ml0.2 M Tris buffer, pH 7.4 2.5 ml0.5 M cobalt chloride 0.5 ml0.05 M magnesium chloride 1.0 mlDistilled water 2.5 mlThe pH of this solution is adjusted to 7.0 if necessary. Aliquot and store at -20°C until required. Sample PreparationCut cytostat sections at 5 to 7 microns. Do not fix. Method:1. Incubate sections in appropriate incubation solution at 37°C for 30-60 minutes.2. Transfer sections to 15% formol saline, 15 minutes.3. Wash in distilled water4. Counterstain in 2% methyl green if required.5. Wash in distilled water.6. Mount in glycerin Jelly. ResultsEnzyme: Black formazan deposits with MTT Source:  Bancroft and Gamble, Theory and Practice of Histological Techniques, Fifth Edition. Churchill Livingstone, London, 2002, page 610.

      Helpful?

  5. Why is a reference wavelength used for data analysis of Thiazolyl Blue Tetrazolium Bromide (MTT)?

    1 answer

    1. The reference wavelength (630-690 nm) is used to adjust for the optical variation in the wells of the plate.  It also corrects for dust and fingerprints that can be present.  The assay is read at 570 nm.  The reference wavelength needs to be far enough away from the assay wavelength so as not to affect those values.

      Helpful?

  6. What can be used to make a solution of Thiazolyl Blue Tetrazolium Bromide (MTT)?

    1 answer

    1. We test the solubility of MTT in water at a concentration of 5 mg/mL.  For the MTT viability assay, we dissolve Product No. M5655 at a concentration of 5 mg/ml in RPMI-1640 without phenol red. This medium is available as a powder (Product No.R8755) or liquid (Product No. R7509). MTT can be solubilized in any culture media or balanced salt solution that does not contain phenol red as a pH indicator.

      Helpful?

  7. How can I store solutions of Thiazolyl Blue Tetrazolium Bromide (MTT)?

    1 answer

    1. Reconstituted MTT solution is stable for at least 6 months when stored frozen (-20 °C). Storage of reconstituted MTT solution at 2-8°C for more than 2 weeks may cause decomposition and yield erroneous results.

      Helpful?

  8. What type of multi-well plate should be used for the Thiazolyl Blue Tetrazolium Bromide (MTT) assay?

    1 answer

    1. It is recommended that flat bottom multi-well plates be used for the MTT assay. The use of tissue culture treated plates is only important if necessary for good cell growth.  If cells are grown in larger plates, the supernatant after cell lysis can be transferred to a 96 well plate for reading.

      Helpful?

  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  10. Is the MTT assay quantitative?

    1 answer

    1. The MTT asay is a method to assess cell viability.  This assay is a semiquantitative assay. The assay is used to compare the viability changes in treated cells to untreated cells. The absorbance is indicative of the cell number. The higher the absorbance, the greater the number of viable cells present. Most researchers compare the absorbance of the two samples as a ratio (ABS treated cells/ABS untreated cells) to get a fold increase/decrease in cell number.

      Helpful?

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