H23830

HEPES

≥99% (titration), crystalline, reagent grade

• 别名: 4-（2-羟乙基）哌嗪-1-乙磺酸, N-（2-羟乙基）哌嗪-N′-（2-乙磺酸）
• 经验公式（希尔记法）: C₈H₁₈N₂O₄S
• CAS号: 7365-45-9
• 分子量: 238.30
• Beilstein: 883043
• MDL號碼: MFCD00006158
• 分類程式碼代碼: 12161700
• NACRES: NA.25

属性

• product name: HEPES, reagent grade, ≥99% (titration), crystalline
• 生物源: synthetic
• 品質等級: 200
• 等級: reagent grade
• 化驗: ≥99% (titration)
• 形狀: crystalline
• 儲存條件: (Tightly closed. Dry)
• 雜質: ≤1% water
• 顏色: white
• 有用的pH值範圍: 6.8-8.2
• pKa (25 °C): 7.5
• mp: 212.6 °C
• 溶解度: water: 1 M, clear, colorless
• 密度: 1.44 g/cm³ at 20 °C
• 正離子痕跡: Fe: <5 ppm
• 儲存溫度: room temp
• SMILES 字串: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI 密鑰: JKMHFZQWWAIEOD-UHFFFAOYSA-N

说明

一般說明

HEPES, or 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, serves as a zwitterionic N-substituted aminosulfonic acid buffer with exceptional buffering capacity (pH 6.8-8.2, pKa 7.48 at 25°C). Recognized for versatility, it is extensively employed in cell biology, biochemical, and biological research. Its noteworthy feature lies in its neutrality towards metal ions, making it a preferred "non-coordinating buffer" suitable for solutions containing metals without affecting their activity. In cell biology studies, HEPES excels at maintaining stable physiological pH despite carbon dioxide fluctuations. It outperforms bicarbonate buffers commonly used in this context.

HEPES extends its utility as a preferred separator in creating pH gradients for isoelectric focusing, a technique in protein separation. Beyond these applications, it proves beneficial in immunoprecipitation, cell lysis, live cell imaging, establishing its indispensability across diverse research areas due to its versatility and minimal interaction with other molecules.

應用

HEPES has been used:

• as a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo
• as a component of Hanks′ solution used in the degradation testing of Iron
• as a component of HEPES medium in the transfection of cell culture with plasmids
• along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells
• as a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies

特點和優勢

• High purity product for biochemical and biological research
• Highly soluble in water with a useful pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C

• Broad buffer range between pH 6.8 to 8.2
• Zwitterionic at biological pH
• High solubility and stability
• Low UV and visible light spectrum absorbance
• Negligible metal ion binding
• Suitable for cell culture, electrophoresis, in vitro methods, etc.

其他說明

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安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable