Solutions of Fast Green FCF are used much like solutions of Light Green SF Yellowish. The advantage of using Fast Green FCF is generally that the stain is more intense. The working solution is typically prepared fresh each day or used for a short period of time.
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25 G
$324.60
About This Item
线性分子式:
C37H34N2O10S3Na2
CAS号:
分子量:
808.85
顏色索引編號:
42053
Beilstein:
5718212
EC號碼:
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.47
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等級
certified by the Biological Stain Commission
品質等級
形狀
powder
成份
dye content, ≥90%
mp
290 °C (dec.) (lit.)
溶解度
H2O: 1 mg/mL
應用
diagnostic assay manufacturing
hematology
histology
儲存溫度
room temp
SMILES 字串
[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O
InChI
1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
InChI 密鑰
RZSYLLSAWYUBPE-UHFFFAOYSA-L
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應用
固绿 FCF 染料用于 IEF 中的蛋白质染色，天然 PAGE 和 SDS-PAGE。 固绿染色在比亮蓝 R 更广泛的蛋白质浓度范围内呈线性。为了使灵敏度最高，建议电泳后将蛋白质固定在凝胶中。 然后用 0.1％ 固绿 FCF 在 30％（v/v）乙醇，10％（v/v）乙酸或 7％ 乙酸中将凝胶染色 2 小时。 凝胶可以用 30％（v/v）乙醇，10％（v/v）乙酸或 7％ 乙酸脱色。 可以在 625nm 处扫描染色的凝胶。 检测灵敏度约为亮蓝 R 的 30％。
適合性
番红固绿 FCF 植物细胞染色剂认证；作为 Gomori一步三色程序的苯胺蓝替代品。
訊號詞
Warning
危險聲明
危險分類
Muta. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
閃點（°F）
352.4 °F - closed cup
閃點（°C）
178 °C - closed cup
個人防護裝備
Eyeshields, Gloves
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What is the proper method to prepare Fast Green FCF for use as a counterstain?
1 answer-
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Looking for a green dye to add to electrolyzed water. Something biodegradable that will disappear fast on skin.
1 answer-
It is advised to source a FD&C (Food, Drug & Cosmetic) grade dye for use on human skin. Assuming there is suitable electrolyzed water, green food coloring should suffice for the intended purpose. The duration for which the dye remains on the skin would depend on factors such as the pH of the water used and the specific characteristics of the dye, which the customer would need to determine.
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