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Merck

861251

Sigma-Aldrich

中性红

certified by the BSC

别名:

3-氨基-6-二甲氨基-2-甲基吩嗪 盐酸盐, 二苯乙烯红, 碱性红 5

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25 G
$297.00

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25 G
$297.00

About This Item

经验公式(希尔记法):
C15H17ClN4
CAS号:
分子量:
288.78
颜色索引号:
50040
Beilstein:
3918740
EC 号:
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.47

$297.00


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Agency

certified by the BSC

质量水平

表单

powder

组成

Dye content, 50-60%

技术

titration: suitable

pH值(酸碱度)

6.8-8.0, red to yellow

mp

290 °C (dec.) (lit.)

溶解性

water: 1 mg/mL

λmax

540 nm

应用

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES字符串

Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1

InChI

1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H

InChI key

PGSADBUBUOPOJS-UHFFFAOYSA-N

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应用

中性红已被用作微浮游生物和中浮游生物的染色剂。[1] 它已被用作刺丝胞的染色剂。[2]
用作制备中性红试纸时的指示剂,以及用作生物染色剂。

生化/生理作用

中性红可用作制备中性红纸的指示剂和生物染料。中性染料毒性低,是一种重要的染料。它也可用作pH指示剂。[3]它可用于细胞活力测定。染料会在健康的活细胞溶酶体中积累,但不会在受损的细胞中积累。[4]

适用性

经认证用于活的血细胞的体外活体染色的Doan & Ralph方法。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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The Anatomical Record, 27, 241-241 (1924)
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Zetsche EV and Meysman FJ
Journal of Plankton Research, 34, 493-509 (2012)
Clung,C.E., Ed.
Handbook of Microscopical Technique, 571-571 (1950)
Catostylus tagi: partial rDNA sequencing and characterisation of nematocyte structures using two improvements in jellyfish sample preparation.
Parracho T and Morais Z
Journal of Venomous Animals and Toxins including Tropical Diseases, 21, 40-40 (2015)

Questions

1–2 of 2 Questions  
  1. How to prepare Neutral Red 861251 for staining dinoflagellates?

    1 answer
    1. For staining dinoflagellates using Neutral Red powder, it is recommended to use the same basic formulation as the Neutral Red solution offered as catalog number N2889, which is stated to be 3.3 g/L dissolved in a DPBS. It should be possible to dissolve in any suitable cell culture media that does not contain phenol red in the formulation. Neutral Red is a vital stain, so the dye needs to be dissolved into an isotonic solution and should not be dissolved in water.

      Helpful?

  2. What percentage is this neutral red solution?

    1 answer
    1. The dye content specification for this product is 50 - 60% Neutral Red. The exact percentage is lot-specific and will be reported on the Certificate of Analysis. See the link below for a sample Certificate of Analysis:
      https://www.sigmaaldrich.com/coa/SIAL/861251/11714LE

      Helpful?

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