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Merck

TOX4

Sigma-Aldrich

体外毒理学检测试剂盒,基于中性红

别名:

NR uptake assay, Neutral red assay

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.84

形狀

liquid

用途

 kit sufficient for 1,000 tests

包裝

pkg of 1 kit

儲存條件

dry at room temperature

λmax

540 nm

應用

cell analysis
detection

檢測方法

colorimetric

儲存溫度

2-8°C

一般說明

用于分光光度法活细胞测定。在540 nm波长下检测转化染料的吸光度。

應用

基于中性红的体外毒理检测试剂盒已用于
  • 测定HB8902细胞内溶酶体酶活性
  • 研究人肺泡上皮细胞的细胞活力
  • 研究成纤维细胞的细胞活力

生化/生理作用

中性红(NR)是一种红色染料,通过细胞膜主动转移在溶酶体中积累。染料被提取并根据光谱定量吸收。NR摄取试验是最常用的细胞毒性试验。它广泛应用于环境和生物医学研究中,用于估计培养中的活细胞数量。NR染料体外组织培养研究用于评估病毒细胞致病性和检测有毒化合物。

訊號詞

Danger

危險聲明

危險分類

Carc. 1B - Flam. Liq. 3 - Skin Sens. 1

儲存類別代碼

3 - Flammable liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

73.4 °F

閃點(°C)

23 °C


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