跳转至内容
Merck
所有图片(1)

主要文件

查看所有文档

ENDOLYSS-RO

Roche

蛋白内切酶Lys-C 测序级

from Lysobacter enzymogenes

别名:

lys-c, 蛋白酶, 赖氨酸内肽酶

登录查看公司和协议定价

选择尺寸

5 μG
$467.00
3 X 5 μG
$1,320.00

$467.00

请联系客服了解存货情况
所有图片(1)

选择尺寸

变更视图
5 μG
$467.00
3 X 5 μG
$1,320.00

About This Item

EC 号:
3.4.21.50 (BRENDA, IUBMB)
UNSPSC代码:
12352204

$467.00

请联系客服了解存货情况

生物来源

bacterial (Lysobacter enzymogenes)

表单

lyophilized

比活

≥200 units/mg protein (at 25 °C, with Chromozym PL)

分子量

33 kDa by SDS-PAGE (reducing)

纯化方式

electrophoresis

包装

pkg of 3 × 5 μg (11047825001)
pkg of 5 μg (11420429001)

制造商/商品名称

Roche

储存条件

(Keep container tightly closed in a dry and well-ventilated place.)

技术

activity assay: suitable

颜色

white

最佳pH

8.5-8.8

pH值范围

5-12

溶解性

water: soluble

适用性

suitable for enzyme test

UniProt登记号

Q7M135

储存温度

2-8°C

相关类别

比较类似商品

查看完整比较结果

显示差异

1 of 4

此商品
ENDOGLUS-ROENDOARGS-RO11418467001
蛋白内切酶Lys-C 测序级 from Lysobacter enzymogenes

ENDOLYSS-RO

蛋白内切酶Lys-C 测序级

胞内蛋白酶 Glu-C 测序级 from Staphylococcus aureus V8

ENDOGLUS-RO

胞内蛋白酶 Glu-C 测序级

胞内蛋白酶 Asp-N 测序级 from Pseudomonas fragi

ENDOARGS-RO

胞内蛋白酶 Asp-N 测序级

胰凝乳蛋白酶，测序级 from bovine pancreas

11418467001

胰凝乳蛋白酶，测序级

grade

protein sequencing grade

grade

-

grade

-

grade

-

specific activity

≥200 units/mg protein (at 25 °C, with Chromozym PL)

specific activity

≥15 units/mg protein

specific activity

≥20000 units/mg protein

specific activity

≥70 units/mg protein (at 25 °C, with ATEE as a substrate)

technique(s)

activity assay: suitable

technique(s)

-

technique(s)

-

technique(s)

protein sequencing: suitable

biological source

bacterial (Lysobacter enzymogenes)

biological source

Staphylococcus aureus (V8)

biological source

-

biological source

bovine pancreas

suitability

suitable for enzyme test

suitability

-

suitability

-

suitability

-

form

lyophilized

form

lyophilized (salt-free)

form

lyophilized

form

lyophilized (salt-free)

一般描述

内蛋白酶赖氨酸-碳测序等级是从产酶溶血杆菌中分离出来的，是一种高度纯化和特异的蛋白酶。来自产酶溶杆菌的内切蛋白酶 Lys-C是肽酶S1家族的一员。它主要用于蛋白质序列分析中多肽链的初始断裂。[1]

特异性

这种酶是一种丝氨酸蛋白酶，能特异性水解赖氨酸羧基侧的酰胺、酯和肽键（在三-盐酸缓冲液中，pH 7.0至9.0）。
热失活：酶可以通过加热（100℃下5分钟）或TCA变性。

应用

使用内蛋白酶赖氨酸-碳测序等级进行蛋白质结构分析和序列分析。它适用于消化溶液、聚丙烯酰胺凝胶或吸水膜中的蛋白质。[2][3][4][5]

制备说明

储存条件（工作溶液）：+2至+8℃

重悬

冻干内蛋白酶赖氨酸-碳测序等级在50 & # 956；L双蒸水中重构。生成缓冲液浓度为50 mM Hepes、pH 8.0、10 mM乙二胺四乙酸和5 mg/ml棉子糖。为避免自溶，培养温度不应超过37℃。
在4 M尿素中稳定。

其他说明

仅用于生命科学研究。不可用于诊断。

储存分类代码

11 - Combustible Solids

WGK

WGK 2

闪点(°F)

does not flash

闪点(°C)

does not flash

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
71452525
71452524
71452523
71452522
71452521

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Heterologous expression and pro-peptide supported refolding of the high specific endopeptidase Lys-C
Stressler T, et al.
Protein Expression and Purification, 118, 31-38 (2016)
H Alexander Ebhardt et al.
Rapid communications in mass spectrometry : RCM, 28(24), 2735-2743 (2014-11-08)
Tandem mass (MS/MS) spectra generated by collision-induced dissociation (CID) typically lack redundant peptide sequence information in the form of e.g. b- and y-ion series due to frequent use of sequence-specific endopeptidases cleaving C- or N-terminal to Arg or Lys residues.
Tao Xu et al.
Nature protocols, 4(3), 325-332 (2009-02-21)
This protocol outlines the essential steps of mass spectrometry-based analysis of protein samples that can be used to identify post-translational arginylation. We describe special considerations for sample preparation and digestion, mass spectrometry analysis using high-precision instruments, database searching for the
Jung Hun Oh et al.
Journal of proteome research, 10(3), 1406-1415 (2011-01-14)
Many efforts have been made to discover novel bio-markers for early disease detection in oncology. However, the lack of efficient computational strategies impedes the discovery of disease-specific biomarkers for better understanding and management of treatment outcomes. In this study, we
Jason A Wall et al.
American journal of physiology. Heart and circulatory physiology, 291(5), H2462-H2472 (2006-06-13)
Ischemia-reperfusion (I/R) has critical consequences in the heart. Recent studies on the functions of I/R-activated kinases, such as p38 mitogen-activated protein kinase (MAPK), showed that I/R injury is reduced in the hearts of transgenic mice that overexpress the p38 MAPK

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持