$1,430.00
请联系客服了解存货情况
推荐产品
用途
sufficient for ≤1,000 tests
质量水平
制造商/商品名称
Roche
技术
ELISA: suitable
检测方法
chemiluminescent
运输
wet ice
储存温度
2-8°C
一般描述
通过测量DNA合成期间的BrdU掺入而定量细胞增殖的化学发光免疫分析方法： [3H]-胸苷掺入测定的非放射性替代方法。
特异性
抗体结合物与胸苷类似物5-溴-2′-脱氧尿苷（BrdU）以及掺入DNA的BrdU反应。为结合掺入DNA的BrdU，必须使BrdU标记的DNA变性。抗体不与胸苷、尿苷或DNA等任何内源性细胞组分发生交叉反应。
应用
细胞增殖ELISA，BrdU（化学发光法）属于第二代改良型DNA合成检测试剂盒。它是一个精确、快速且简单的非放射性替代方法，通过测量复制（循环）细胞中DNA合成期间的BrdU掺入而定量细胞增殖。因此，细胞增殖ELISA可用于许多不同的 体外 细胞系统。例如：
包装
1个试剂盒包含7种组分。
组分
- BrdU 标记试剂 1,000倍 浓度
- FixDenat
- 抗-BrdU-POD 4.抗体稀释溶液
- PBS 洗涤缓冲溶液, 10倍浓度
- 底物组分 A
- 底物组分 B
其他说明
仅用于生命科学研究。不可用于诊断。
仅试剂盒组分
产品编号
说明
- BrdU Labeling Reagent
- FixDenat ready-to-use
- Anti-BrdU-peroxidase antibody
- Antibody Dilution Solution ready-to-use
- ready-to-use 10x concentrated
- Substrate Component A
- Substrate Component B
警示用语：
Danger
危险分类
Eye Irrit. 2 - Flam. Liq. 2 - Muta. 1B - Skin Sens. 1
储存分类代码
3 - Flammable liquids
WGK
WGK 1
其他客户在看
Valerio Ciccone et al.
Oncotarget, 9(17), 13353-13365 (2018-03-24)
Nitric oxide (NO) exerts conflicting effect on tumor growth and progression, depending on its concentration. We aimed to characterize the anti-cancer activity of a new NO donor, Ni(SalPipNONO) belonging to the class of metal-nonoates, in epithelial derived tumor cells, finally
Xiao-Feng Wang et al.
Molecular medicine reports, 12(4), 5917-5923 (2015-08-25)
Epirubicin, an anthracycline derivative, is one of the main line treatments for brain tumors. The aim of the present study was to verify that epirubicin alters the growth and morphological characteristics of U‑87 glioma cells. In the present study, the
Diego S D'Astolfo et al.
Cell, 161(3), 674-690 (2015-04-25)
Modulation of protein function is used to intervene in cellular processes but is often done indirectly by means of introducing DNA or mRNA encoding the effector protein. Thus far, direct intracellular delivery of proteins has remained challenging. We developed a
Juliann E Kosovec et al.
Oncotarget, 8(59), 100421-100432 (2017-12-17)
Esophageal adenocarcinoma (EAC) is a deadly disease with limited therapeutic options. In the present study, we determined the preclinical efficacy of CDK4/6 inhibitor abemaciclib for treatment of EAC. In vitro, apoptosis, proliferation, and pathway regulation were evaluated in OE19, OE33
Caixia An et al.
International journal of oncology, 54(4), 1195-1208 (2019-04-11)
Emerging studies have reported that coatomer protein complex subunit β2 (COPB2) is overexpressed in several types of malignant tumor; however, to the best of our knowledge, no studies regarding COPB2 in gastric cancer have been published thus far. Therefore, the present study
商品
Cell cycle regulates vital processes like DNA repair, cancer prevention. Four stages: G1, S, G2, M. NTPs don't permeate membranes.
基于细胞的细胞增殖检测（BrdU、MTT、WST1）、细胞活力和细胞毒性实验，用于癌症、神经科学和干细胞研究应用。
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持
