推荐产品
生物来源
sheep
质量水平
偶联物
peroxidase conjugate
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
polyclonal
表单
lyophilized
包装
pkg of 50 U
制造商/商品名称
Roche
同位素/亚型
IgG
储存温度
2-8°C
一般描述
特异性
来自绵羊的多克隆抗体对地高辛配基和地高辛具有特异性,并且与其他类固醇(如人类雌激素和雄激素)没有交叉反应性。
应用
制备说明
工作浓度:结合物的工作浓度取决于应用类型和底物。以下浓度可作为指导:
工作溶液: 100mM Tris-HCl,150mM NaCl,pH 7.5。
1%封闭试剂(w/v),1至5%热灭活的胎牛血清(v/v)或绵羊正常血清(可用于减少非特异性结合)。
- 斑点印迹:50至100mU/ml
- ELISA:2至50mU/ml
- 蛋白质印迹:50至100mU/ml
- Southern印迹:50至100mU/ml
工作溶液: 100mM Tris-HCl,150mM NaCl,pH 7.5。
1%封闭试剂(w/v),1至5%热灭活的胎牛血清(v/v)或绵羊正常血清(可用于减少非特异性结合)。
重悬
加入1ml双蒸水至终浓度为50U/ml。
其他说明
仅用于生命科学研究。不可用于诊断。
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警示用语:
Warning
危险声明
危险分类
Skin Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
does not flash
闪点(°C)
does not flash
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Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
地高辛(DIG)标记方法和试剂盒适用于DNA和RNA DIG探针、随机引物DNA标记、切口平移标记、5'和3'寡核苷酸末端标记。
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