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Merck

11388908910

Roche

生物素-16-UTP

pkg of 25 μL (250 nmol; 10mM)

别名:

生物素-16-UTP, 生物素

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25 μL
$685.00

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25 μL
$685.00

About This Item

UNSPSC代码:
41116100

$685.00


请联系客服了解存货情况

方案

97.7% (HPLC)

质量水平

表单

solution

分子量

Mr 987.5 (biotin-16-UTP-Li4)

包装

pkg of 25 μL (250 nmol; 10mM)

制造商/商品名称

Roche

颜色

colorless

溶解性

water: miscible

运输

dry ice

储存温度

−20°C

一般描述

生物素-16-UTP中的生物素通过酰胺键与尿苷三磷酸结合。生物素-16-UTP是SP6、T3和T7 RNA聚合酶的底物。它可以取代体外转录反应中的UTP,用于RNA标记。[1]利用ATP、GTP、CTP、UTP和生物素-16-UTP和相应的RNA聚合酶分别对具有T7、SP6或T3启动子的线性模板DNA进行体外转录。

应用

生物素标记的RNA可作为杂交探针用于:
  • Northern 印迹
  • Southern 印迹
  • 菌落/菌斑转移筛选
  • RNase保护实验
  • 原位杂交
  • 芯片杂交[2][3]
随后,使用荧光链霉亲和素结合物检测标记的RNA,或使用链霉亲和素-AP结合物通过酶联免疫吸附测定(ELISA)检测标记的RNA。

质量

典型分析:至少85%生物素-16-UTP(HPLC,面积%)。

外形

生物素-16-UTP,四锂盐,10mM溶液

其他说明

仅用于生命科学研究。不用于诊断操作。

WGK

WGK 1

闪点(°F)

does not flash

闪点(°C)

does not flash


历史批次信息供参考:

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