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Merck
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主要文件

UFC9003

Millipore

Amicon® Ultra过滤器，3 kDa MWCO

greener alternative

sample volume 15 mL, regenerated cellulose membrane, MWCO 3 kDa

别名:

离心浓缩器，配备Ultracel®膜，3 kDa 截留分子量，15 mL样品体积

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About This Item

UNSPSC代码:
41104916
eCl@ss:
36100101
NACRES:
NB.24
价格与库存信息目前不能提供

物料

polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing

质量水平

400

无菌性

non-sterile

产品线

Amicon®

制造商/商品名称

Amicon® Ultra

sustainability

Greener Alternative Product

参数

15 mL sample volume

技术

protein extraction: suitable
protein purification: suitable

长度

12.1 cm (4.807 in.)

直径

2.97 cm

过滤面积

7.6 cm2

体积

15 mL

工作体积

15 mL

孔径

3 kDa MWCO

环保替代产品分类

Re-engineered

运输

ambient

相关类别

一般描述

配备截留分子量3,000道尔顿Ultracel ®过滤器的 Amicon ®Ultra 15 mL 离心设备实现快速的超滤和大分子的可靠截留。例如，初始浓度为 0.25 mg/ml 的细胞色素 C（12.4 kDa）截留率为 >90%。

垂直设计和可用膜表面积实现了快速的样品处理、高样品回收率（稀释起始溶液通常大于 90% ）和 80 倍浓缩能力。垂直设计可最大程度减少溶质极化和随后的膜污染，过滤设备中的物理止动装置（physical deadstop）防止旋转至干燥和潜在的样品损失。

用移液管从过滤设备样品储罐中收集浓缩物，将超滤液收集在随配的离心管中。该设备可以在摇摆斗或固定角转子中旋转。Amicon ®Ultra 15 mL 过滤器采用垂直膜设计和止动装置（deadstop），可在较短的旋转时间内达到最高的回收率。
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.

应用

  • 采用截留分子量为 3,000 道尔顿的 Ultracel ®膜进行超滤。
  • 浓缩含有抗原、抗体、酶、核酸或微生物的生物样品。
  • 纯化组织培养提取物或细胞裂解物中的大分子成分，在 HPLC 之前去除蛋白质。
  • 浓缩柱洗脱液中的稀释或预纯化的蛋白质。
  • 脱盐、缓冲液交换和蛋白质透析。

特点和优势

  • 截留分子量（NMWCO）为 3 kDa 的 Ultracel ®再生纤维素膜可实现大于 90% 的截留物回收率。
  • 垂直膜减少了浓度极化，实现超快旋转时间（快至 10-15 分钟）。
  • 热封膜可最大程度减少下游可萃取物。
  • 100% 经过完整性测试，性能可靠。
  • 半透明外壳和体积刻度方便样品监测。
  • 移液器直接移取样品消除了回收浓缩物的处理步骤。
  • 80–100X的高浓缩因子。

外形

色码：透明，带有粉色盖

法律信息

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

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蛋白样品超滤

本页内容讨论了使用超滤膜进行蛋白样品制备的原理和优势。应用包括溶剂置换、蛋白样品浓缩或纯化。

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相关内容

Amicon® Ultra离心超滤管

浓缩你的蛋白质，别失去它。使用Amicon® Ultra过滤器进行浓缩，能可靠回收蛋白质。

Questions

1–7 of 7 Questions  

  1. Can we reuse the tube or it is single time use only?

    1 answer

    1. This product is intended for single use. Repeated usage may result in declining performance.

      Helpful?

  2. Hello, I did not get if all the proteins will remain in the filter and if I can recover them from the filter (product: UFC9003 Millipore Amicon® Ultra Centrifugal Filter, 3 kDa MWCO). What will exactly remain in the liquid part in the bottom? Many thanks

    1 answer

    1. These centrifugal devices separate constituents of a given sample by molecular weight. This product has a molecular weight cut-off (MWCO) of 3000 Daltons. Components of the sample with a molecular weight greater that the designated 3 kDa will remain in the retained portion following centrifugation, while components of less that 3 kDa will filter through. See the image below to review this 2 part device.
      Please see the following link to review the product User Guide:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/395/pr05035w-ug-ms.pdf

      The link below is a video demonstrating the practical use of the Amicon products:
      https://www.youtube.com/watch?v=7uUUYqIpI6U

      Helpful?

  3. Hi, I am writing to ask if you have centrifugal filters with hydrophobic membranes. I am looking to test them for oil/water separation downstream to a fermentation process for food applications.

    1 answer

    1. The ultrafiltration membranes used in Amicon devices are all hydrophilic. There are no hydrophobic options.

      Helpful?

  4. Even sanitizing the devices with 70% ethanol, how many times may I use one tube? I mean, How many times can be used it?

    1 answer

    1. The unit is for one-time use only.

      Helpful?

  5. How many tubes are inside the pack? For example, there is a pack size of 8 pkg. What does it mean? Does it mean 8 tubes or 8 packs of tubes? Does it mean that there are 8 tubes in the pack? Can you explain more, please?

    1 answer

    1. The package quantity for each item is as follows:
      UFC900308 - 8 tubes
      UFC900324 - 24 tubes
      UFC900396 - 96 tubes

      Helpful?

  6. How much g (rcf) force can be applied during the spin?

    1 answer

    1. Amicon® Ultra-15 Centrifugal Filter Devices can be spun at 4,000 x g maximum for approximately 15-60 minutes when using a swinging-bucket rotor, or at 5,000 x g maximum for approximately 15-60 minutes when using a fixed-angle rotor.

      Helpful?

  7. Is autoclave sterilization possible?

    1 answer

    1. The Amicon Ultra devices are manufactured under very thorough and controlled cleaning conditions, however they are not sterile and cannot be sterilized by UV or autoclaving. However, it is possible to sanitize the devices with 70% ethanol, followed by several rinses with sterile water or culture medium.

      Helpful?

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